Transcriptome and Metabolome Analysis of BmFAMeT6 Overexpression in Bombyx mori
Abstract
:1. Introduction
2. Material and Methods
2.1. Insects and Environment
2.2. Experiment Design and Material Collection
2.3. Total RNA Isolation, Preparation, and Sequencing of Digital Gene Expression (DGE) Library
2.4. Metabolite Detection and Differential Metabolite Identification
2.5. Statistical Analysis of Data
3. Results
3.1. Overexpression of BmFAMeT6 Affected Gene Differential Expression in Silkworm Larvae
3.2. The Overexpression of BmFAMeT6 Mainly Affects the Molecular Functions and Biological Processes of Silkworm Larvae
3.3. BmFAMeT6 May Modulate Larval Metabolic Pathways through Zinc Finger Transcription Factors
3.4. Overexpression of BmFAMeT6 Led to Metabolic Differences in the Silkworm Larvae
3.5. The Differential Metabolites Caused by BmFAMeT6
3.6. The Main Metabolism Pathway and the Same Differential Metabolite by Overexpression of BmFAMeT6
4. Discussion
4.1. The Regulatory Role of BmFAMeT6 Is Carried Out in the Context of Growth and Development
4.2. Differential Gene Enrichment in the Metabolic Pathway Indicates That BmFAMeT6 May Be Involved in the Balance between Growth and Detoxification Metabolism
4.3. The Zinc Finger C2H2 (zf-C2H2) Transcription Factor May Have Made an Important Contribution to the Regulation of BmFAMeT6
4.4. The BmFAMeT6 May Influence the Metabolism of Silkworm Larvae by Participating in the ABC Transporters, Purine Metabolism Pathway, and Tyrosine Metabolism
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yu, Y.; Li, T.; Chen, P. Transcriptome and Metabolome Analysis of BmFAMeT6 Overexpression in Bombyx mori. Genes 2024, 15, 1261. https://doi.org/10.3390/genes15101261
Yu Y, Li T, Chen P. Transcriptome and Metabolome Analysis of BmFAMeT6 Overexpression in Bombyx mori. Genes. 2024; 15(10):1261. https://doi.org/10.3390/genes15101261
Chicago/Turabian StyleYu, Yang, Tian Li, and Ping Chen. 2024. "Transcriptome and Metabolome Analysis of BmFAMeT6 Overexpression in Bombyx mori" Genes 15, no. 10: 1261. https://doi.org/10.3390/genes15101261
APA StyleYu, Y., Li, T., & Chen, P. (2024). Transcriptome and Metabolome Analysis of BmFAMeT6 Overexpression in Bombyx mori. Genes, 15(10), 1261. https://doi.org/10.3390/genes15101261