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Article

miR-499a rs3746444 A>G Polymorphism Is Correlated with Type 2 Diabetes Mellitus and Diabetic Polyneuropathy in a Romanian Cohort: A Preliminary Study

by
Emilia Burada
1,2,†,
Maria-Magdalena Roșu
3,†,
Raluca Elena Sandu
2,4,
Florin Burada
5,6,*,
Mihai Gabriel Cucu
5,6,†,
Ioana Streață
5,6,
Bianca Petre-Mandache
5,7,
Gabriela Popescu-Hobeanu
5,7,
Monica-Laura Cara
8,
Anca-Maria Țucă
1,7,
Elena Pinoșanu
2,7 and
Carmen Valeria Albu
2,9
1
Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
2
Department of Neurology, Clinical Hospital of Neuropsychiatry Craiova, 200473 Craiova, Romania
3
Department of Diabetes, Nutrition and Metabolic Diseases, Emergency Clinical County Hospital Craiova, 200642 Craiova, Romania
4
Department of Biochemistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
5
Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, 200638 Craiova, Romania
6
Regional Centre of Medical Genetics Dolj, Emergency Clinical County Hospital Craiova, 200642 Craiova, Romania
7
Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
8
Department of Public Health, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
9
Department of Neurology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Genes 2023, 14(8), 1543; https://doi.org/10.3390/genes14081543
Submission received: 6 July 2023 / Revised: 20 July 2023 / Accepted: 25 July 2023 / Published: 27 July 2023
(This article belongs to the Section RNA)
Review Reports Versions Notes

Abstract

Type 2 diabetes mellitus (T2DM) is a common metabolic disorder that results from complex interactions of both environmental and genetic factors. Many single nucleotide polymorphisms (SNPs), including noncoding RNA genes, have been investigated for their association with susceptibility to T2DM and its complications, with little evidence available regarding Caucasians. The aim of the present study was to establish whether four miRNA SNPs (miR-27a rs895819 T>C, miR-146a rs2910164 G>C, miR-196a2 rs11614913 C>T, and miR-499a rs3746444 A>G) are correlated with susceptibility to T2DM and/or diabetic polyneuropathy (DPN) in a Romanian population. A total of 167 adult T2DM patients and 324 age- and sex-matched healthy controls were included in our study. miRNA SNPs were detected by real-time PCR using a TaqMan genotyping assay. A significant association with T2DM was observed only for the miR-499a rs3746444 A>G SNP in all the tested models, and the frequencies of both the miR-499a rs3746444 AG and the GG genotypes were higher in the T2DM patients compared to the controls. No correlation was observed for the miR-27a rs895819 T>C, miR-146a rs2910164 G>C, or miR-196a2 rs11614913 C>T SNPs in any genetic model. When we assessed the association of these SNPs with DPN separately, we found a positive association for the miR-499a rs3746444 SNP in both codominant and dominant models (OR 6.47, 95% CI: 1.71–24.47; OR 2.30, 95% CI: 1.23–4.29, respectively). In conclusion, this study shows that miR-499a rs3746444 A>G may influence both T2DM and DPN susceptibility, with carriers of the GG genotype and the G allele being at an increased risk in the Romanian population.
Keywords: type 2 diabetes mellitus; diabetic polyneuropathy; microRNAs; single nucleotide polymorphism; genotype type 2 diabetes mellitus; diabetic polyneuropathy; microRNAs; single nucleotide polymorphism; genotype

Share and Cite

MDPI and ACS Style

Burada, E.; Roșu, M.-M.; Sandu, R.E.; Burada, F.; Cucu, M.G.; Streață, I.; Petre-Mandache, B.; Popescu-Hobeanu, G.; Cara, M.-L.; Țucă, A.-M.; et al. miR-499a rs3746444 A>G Polymorphism Is Correlated with Type 2 Diabetes Mellitus and Diabetic Polyneuropathy in a Romanian Cohort: A Preliminary Study. Genes 2023, 14, 1543. https://doi.org/10.3390/genes14081543

AMA Style

Burada E, Roșu M-M, Sandu RE, Burada F, Cucu MG, Streață I, Petre-Mandache B, Popescu-Hobeanu G, Cara M-L, Țucă A-M, et al. miR-499a rs3746444 A>G Polymorphism Is Correlated with Type 2 Diabetes Mellitus and Diabetic Polyneuropathy in a Romanian Cohort: A Preliminary Study. Genes. 2023; 14(8):1543. https://doi.org/10.3390/genes14081543

Chicago/Turabian Style

Burada, Emilia, Maria-Magdalena Roșu, Raluca Elena Sandu, Florin Burada, Mihai Gabriel Cucu, Ioana Streață, Bianca Petre-Mandache, Gabriela Popescu-Hobeanu, Monica-Laura Cara, Anca-Maria Țucă, and et al. 2023. "miR-499a rs3746444 A>G Polymorphism Is Correlated with Type 2 Diabetes Mellitus and Diabetic Polyneuropathy in a Romanian Cohort: A Preliminary Study" Genes 14, no. 8: 1543. https://doi.org/10.3390/genes14081543

APA Style

Burada, E., Roșu, M.-M., Sandu, R. E., Burada, F., Cucu, M. G., Streață, I., Petre-Mandache, B., Popescu-Hobeanu, G., Cara, M.-L., Țucă, A.-M., Pinoșanu, E., & Albu, C. V. (2023). miR-499a rs3746444 A>G Polymorphism Is Correlated with Type 2 Diabetes Mellitus and Diabetic Polyneuropathy in a Romanian Cohort: A Preliminary Study. Genes, 14(8), 1543. https://doi.org/10.3390/genes14081543

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