Vibrio cholerae Chromosome Partitioning without Polar Anchoring by HubP
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, France
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Academic Editor: Ozgur Bayram
Genes 2022, 13(5), 877; https://doi.org/10.3390/genes13050877
Received: 24 April 2022
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Revised: 10 May 2022
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Accepted: 11 May 2022
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Published: 13 May 2022
(This article belongs to the Special Issue Bacterial DNA Organization and Segregation)
Partition systems are widespread among bacterial chromosomes. They are composed of two effectors, ParA and ParB, and cis acting sites, parS, located close to the replication origin of the chromosome (oriC). ParABS participate in chromosome segregation, at least in part because they serve to properly position sister copies of oriC. A fourth element, located at cell poles, is also involved in some cases, such as HubP for the ParABS1 system of Vibrio cholerae chromosome 1 (ch1). The polar anchoring of oriC of ch1 (oriC1) is lost when HubP or ParABS1 are inactivated. Here, we report that in the absence of HubP, ParABS1 actively maintains oriC1 at mid-cell, leading to the subcellular separation of the two ch1 replication arms. We further show that parS1 sites ectopically inserted in chromosome 2 (ch2) stabilize the inheritance of this replicon in the absence of its endogenous partition system, even without HubP. We also observe the positioning interference between oriC1 and oriC of ch2 regions when their positionings are both driven by ParABS1. Altogether, these data indicate that ParABS1 remains functional in the absence of HubP, which raises questions about the role of the polar anchoring of oriC1 in the cell cycle.
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Keywords:
partition system; chromosome segregation; chromosome organization; HubP; ParABS; Vibrio cholerae
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MDPI and ACS Style
Possoz, C.; Yamaichi, Y.; Galli, E.; Ferat, J.-L.; Barre, F.-X. Vibrio cholerae Chromosome Partitioning without Polar Anchoring by HubP. Genes 2022, 13, 877. https://doi.org/10.3390/genes13050877
AMA Style
Possoz C, Yamaichi Y, Galli E, Ferat J-L, Barre F-X. Vibrio cholerae Chromosome Partitioning without Polar Anchoring by HubP. Genes. 2022; 13(5):877. https://doi.org/10.3390/genes13050877
Chicago/Turabian StylePossoz, Christophe, Yoshiharu Yamaichi, Elisa Galli, Jean-Luc Ferat, and Francois-Xavier Barre. 2022. "Vibrio cholerae Chromosome Partitioning without Polar Anchoring by HubP" Genes 13, no. 5: 877. https://doi.org/10.3390/genes13050877
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