Next Article in Journal
Transcriptome Analysis Reveals Differentially Expressed Genes That Regulate Biosynthesis of the Active Compounds with Methyl Jasmonate in Rosemary Suspension Cells
Previous Article in Journal
LRRTM4 Terminal Exon Duplicated in Family with Tourette Syndrome, Autism and ADHD
Article

Genome-Wide Identification of Key Components of RNA Silencing in Two Phaseolus vulgaris Genotypes of Contrasting Origin and Their Expression Analyses in Response to Fungal Infection

1
Université Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91405 Orsay, France
2
Université de Paris, Institute of Plant Sciences Paris Saclay (IPS2), 91405 Orsay, France
3
Molecular Plant Pathology, Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, 1098 XH Amsterdam, The Netherlands
4
Laboratoire de Mathématiques et Modélisation d’Evry, Université Paris-Saclay, CNRS, Université Evry, INRAE, 91037 Evry, France
*
Author to whom correspondence should be addressed.
Equal contributions were made by A.G. and V.G., who should be considered joint last authors.
Academic Editor: Frank L. W. Takken
Genes 2022, 13(1), 64; https://doi.org/10.3390/genes13010064
Received: 3 December 2021 / Revised: 22 December 2021 / Accepted: 23 December 2021 / Published: 27 December 2021
(This article belongs to the Section Plant Genetics and Genomics)
RNA silencing serves key roles in a multitude of cellular processes, including development, stress responses, metabolism, and maintenance of genome integrity. Dicer, Argonaute (AGO), double-stranded RNA binding (DRB) proteins, RNA-dependent RNA polymerase (RDR), and DNA-dependent RNA polymerases known as Pol IV and Pol V form core components to trigger RNA silencing. Common bean (Phaseolus vulgaris) is an important staple crop worldwide. In this study, we aimed to unravel the components of the RNA-guided silencing pathway in this non-model plant, taking advantage of the availability of two genome assemblies of Andean and Meso-American origin. We identified six PvDCLs, thirteen PvAGOs, 10 PvDRBs, 5 PvRDRs, in both genotypes, suggesting no recent gene amplification or deletion after the gene pool separation. In addition, we identified one PvNRPD1 and one PvNRPE1 encoding the largest subunits of Pol IV and Pol V, respectively. These genes were categorized into subgroups based on phylogenetic analyses. Comprehensive analyses of gene structure, genomic localization, and similarity among these genes were performed. Their expression patterns were investigated by means of expression models in different organs using online data and quantitative RT-PCR after pathogen infection. Several of the candidate genes were up-regulated after infection with the fungus Colletotrichum lindemuthianum. View Full-Text
Keywords: Phaseolus vulgaris; Colletotrichum lindemuthianum; RNA silencing; Argonaute; double-stranded RNA binding (DRB); RNA-dependent RNA polymerase (RDR); Pol IV Phaseolus vulgaris; Colletotrichum lindemuthianum; RNA silencing; Argonaute; double-stranded RNA binding (DRB); RNA-dependent RNA polymerase (RDR); Pol IV
Show Figures

Figure 1

MDPI and ACS Style

Alvarez-Diaz, J.C.; Richard, M.M.S.; Thareau, V.; Teano, G.; Paysant-Le-Roux, C.; Rigaill, G.; Pflieger, S.; Gratias, A.; Geffroy, V. Genome-Wide Identification of Key Components of RNA Silencing in Two Phaseolus vulgaris Genotypes of Contrasting Origin and Their Expression Analyses in Response to Fungal Infection. Genes 2022, 13, 64. https://doi.org/10.3390/genes13010064

AMA Style

Alvarez-Diaz JC, Richard MMS, Thareau V, Teano G, Paysant-Le-Roux C, Rigaill G, Pflieger S, Gratias A, Geffroy V. Genome-Wide Identification of Key Components of RNA Silencing in Two Phaseolus vulgaris Genotypes of Contrasting Origin and Their Expression Analyses in Response to Fungal Infection. Genes. 2022; 13(1):64. https://doi.org/10.3390/genes13010064

Chicago/Turabian Style

Alvarez-Diaz, Juan C., Manon M.S. Richard, Vincent Thareau, Gianluca Teano, Christine Paysant-Le-Roux, Guillem Rigaill, Stéphanie Pflieger, Ariane Gratias, and Valérie Geffroy. 2022. "Genome-Wide Identification of Key Components of RNA Silencing in Two Phaseolus vulgaris Genotypes of Contrasting Origin and Their Expression Analyses in Response to Fungal Infection" Genes 13, no. 1: 64. https://doi.org/10.3390/genes13010064

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop