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10.3390/genes13010058
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Article

Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts

by
Ke Xu
1,
Hao Zhou
1,
Chengxiao Han
1,
Zhong Xu
2,
Jinmei Ding
1,
Jianshen Zhu
1,
Chao Qin
1,
Huaixi Luo
1,
Kangchun Chen
1,
Shengyao Jiang
1,
Jiajia Liu
1,
Wenqi Zhu
1 and
He Meng
1,*
1
Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
2
Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Provincial Academy of Agricultural Sciences, Wuhan 430072, China
*
Author to whom correspondence should be addressed.
Genes 2022, 13(1), 58; https://doi.org/10.3390/genes13010058
Submission received: 15 November 2021 / Revised: 12 December 2021 / Accepted: 22 December 2021 / Published: 26 December 2021
(This article belongs to the Section Animal Genetics and Genomics)
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Abstract

In mammals, Myostatin (MSTN) is a known negative regulator of muscle growth and development, but its role in birds is poorly understood. To investigate the molecular mechanism of MSTN on muscle growth and development in chickens, we knocked out MSTN in chicken fetal myoblasts (CFMs) and sequenced the mRNA transcriptomes. The amplicon sequencing results show that the editing efficiency of the cells was 76%. The transcriptomic results showed that 296 differentially expressed genes were generated after down-regulation of MSTN, including angiotensin I converting enzyme (ACE), extracellular fatty acid-binding protein (EXFABP) and troponin T1, slow skeletal type (TNNT1). These genes are closely associated with myoblast differentiation, muscle growth and energy metabolism. Subsequent enrichment analysis showed that DEGs of CFMs were related to MAPK, Pl3K/Akt, and STAT3 signaling pathways. The MAPK and Pl3K/Akt signaling pathways are two of the three known signaling pathways involved in the biological effects of MSTN in mammals, and the STAT3 pathway is also significantly enriched in MSTN knock out chicken leg muscles. The results of this study will help to understand the possible molecular mechanism of MSTN regulating the early differentiation of CFMs and lay a foundation for further research on the molecular mechanism of MSTN involvement in muscle growth and development.
Keywords: Myostatin; CRISPR/Cas9; transcriptome; skeletal muscle; growth and development Myostatin; CRISPR/Cas9; transcriptome; skeletal muscle; growth and development

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MDPI and ACS Style

Xu, K.; Zhou, H.; Han, C.; Xu, Z.; Ding, J.; Zhu, J.; Qin, C.; Luo, H.; Chen, K.; Jiang, S.; et al. Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts. Genes 2022, 13, 58. https://doi.org/10.3390/genes13010058

AMA Style

Xu K, Zhou H, Han C, Xu Z, Ding J, Zhu J, Qin C, Luo H, Chen K, Jiang S, et al. Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts. Genes. 2022; 13(1):58. https://doi.org/10.3390/genes13010058

Chicago/Turabian Style

Xu, Ke, Hao Zhou, Chengxiao Han, Zhong Xu, Jinmei Ding, Jianshen Zhu, Chao Qin, Huaixi Luo, Kangchun Chen, Shengyao Jiang, and et al. 2022. "Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts" Genes 13, no. 1: 58. https://doi.org/10.3390/genes13010058

APA Style

Xu, K., Zhou, H., Han, C., Xu, Z., Ding, J., Zhu, J., Qin, C., Luo, H., Chen, K., Jiang, S., Liu, J., Zhu, W., & Meng, H. (2022). Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts. Genes, 13(1), 58. https://doi.org/10.3390/genes13010058

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