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Article

Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons

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Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
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International Degree Program in Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
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Demin Veterinary Hospital, Kaohsiung 807, Taiwan
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Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, Laguna 4031, Philippines
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Graduate School, University of the Philippines Los Baños, Laguna 4031, Philippines
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School of Chemical, Biological and Materials Engineering and Sciences, Mapúa University, Intramuros, Manila 1002, Philippines
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School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
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Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
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Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan
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Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
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Research Center for Environmental Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan
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Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
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International Program in Ornamental Fish Technology and Aquatic Animal Health, International College, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
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Research Center for Animal Biologics, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
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School of Dentistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan
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Institute for Translation Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 807, Taiwan
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Jae Yong Han
Genes 2021, 12(9), 1383; https://doi.org/10.3390/genes12091383
Received: 25 July 2021 / Revised: 27 August 2021 / Accepted: 31 August 2021 / Published: 3 September 2021
(This article belongs to the Section Animal Genetics and Genomics)
Pigeon racing’s recent upturn in popularity can be attributed in part to the huge prize money involved in these competitions. As such, methods to select pigeons with desirable genetic characteristics for racing or for selective breeding have also been gaining more interest. Polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP) for genotyping-specific genes is one of the most commonly used molecular techniques, which can be costly, laborious and time consuming. The present study reports the development of an alternative genotyping method that employs Kompetitive Allele Specific Polymerase Chain Reaction (KASP) technology with specifically designed primers to detect previously reported racing performance-associated polymorphisms within the LDHA, MTYCB, and DRD4 genes. To validate, KASP assays and PCR-RFLP assays results from 107 samples genotyped for each of the genes were compared and the results showed perfect (100%) agreement of both methods. The developed KASP assays present an alternative rapid, reliable, and cost-effective method to identify polymorphisms in pigeons. View Full-Text
Keywords: DRD4 gene; genetic resources; KASP; LDHA gene; MTCYB gene; PCR-RFLP; polymorphism DRD4 gene; genetic resources; KASP; LDHA gene; MTCYB gene; PCR-RFLP; polymorphism
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MDPI and ACS Style

Chang, C.-C.; Silva, B.B.I.; Huang, H.-Y.; Tsai, C.-Y.; Flores, R.J.D.; Tayo, L.L.; Tyan, Y.-C.; Tsai, M.-A.; Catulin, G.E.M.; Chuang, K.-P.; Yang, J.-L. Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons. Genes 2021, 12, 1383. https://doi.org/10.3390/genes12091383

AMA Style

Chang C-C, Silva BBI, Huang H-Y, Tsai C-Y, Flores RJD, Tayo LL, Tyan Y-C, Tsai M-A, Catulin GEM, Chuang K-P, Yang J-L. Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons. Genes. 2021; 12(9):1383. https://doi.org/10.3390/genes12091383

Chicago/Turabian Style

Chang, Ching-Chi, Benji B.I. Silva, Huai-Ying Huang, Ching-Yi Tsai, Ronilo J.D. Flores, Lemmuel L. Tayo, Yu-Chang Tyan, Ming-An Tsai, Gail E.M. Catulin, Kuo-Pin Chuang, and Jenq-Lin Yang. 2021. "Development and Validation of KASP Assays for the Genotyping of Racing Performance-Associated Single Nucleotide Polymorphisms in Pigeons" Genes 12, no. 9: 1383. https://doi.org/10.3390/genes12091383

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