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Functional Evaluation of Splicing for Variants of Uncertain Significance in Patients with Inherited Retinal Diseases

1
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73114, USA
2
Molecular Vision Laboratories, Hillsboro, OR 97006, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Alejandro Garanto
Genes 2021, 12(7), 993; https://doi.org/10.3390/genes12070993
Received: 8 June 2021 / Revised: 24 June 2021 / Accepted: 25 June 2021 / Published: 29 June 2021
(This article belongs to the Section Human Genomics and Genetic Diseases)
Inherited retinal diseases (IRD) comprise a heterogeneous set of clinical and genetic disorders that lead to blindness. Given the emerging opportunities in precision medicine and gene therapy, it has become increasingly important to determine whether DNA variants with uncertain significance (VUS) are responsible for patients’ IRD. This research was performed to assess the functional consequence of six VUS identified in patients with IRD. Clinical assessments included an ophthalmic examination, best-corrected visual acuity, and kinetic perimetry. Imaging was acquired with the Optos ultra-widefield camera and spectral domain optical coherence tomography (SD-OCT). Genetic testing was performed by Molecular Vision Laboratories. VUS that were predicted to alter splicing were analyzed with a minigene assay, which revealed that VUS in the genes OPA1, CNGB1, and CLUAP1 altered spicing mechanisms. Due to emerging gene and cell therapies, these results expand the genotype-phenotype correlations for patients diagnosed with an IRD. View Full-Text
Keywords: inherited retinal disease; VUS; functional analysis; minigene assay inherited retinal disease; VUS; functional analysis; minigene assay
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MDPI and ACS Style

Mauro-Herrera, M.; Chiang, J.; Radojevic, B.; Bennett, L.D. Functional Evaluation of Splicing for Variants of Uncertain Significance in Patients with Inherited Retinal Diseases. Genes 2021, 12, 993. https://doi.org/10.3390/genes12070993

AMA Style

Mauro-Herrera M, Chiang J, Radojevic B, Bennett LD. Functional Evaluation of Splicing for Variants of Uncertain Significance in Patients with Inherited Retinal Diseases. Genes. 2021; 12(7):993. https://doi.org/10.3390/genes12070993

Chicago/Turabian Style

Mauro-Herrera, Margarita, John Chiang, Bojana Radojevic, and Lea D. Bennett 2021. "Functional Evaluation of Splicing for Variants of Uncertain Significance in Patients with Inherited Retinal Diseases" Genes 12, no. 7: 993. https://doi.org/10.3390/genes12070993

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