Next Article in Journal
De Novo Sporophyte Transcriptome Assembly and Functional Annotation in the Endangered Fern Species Vandenboschia speciosa (Willd.) G. Kunkel
Next Article in Special Issue
Prospects in Connecting Genetic Variation to Variation in Fertility in Male Bees
Previous Article in Journal
Mapping and Analysis of Swi5 and Sfr1 Phosphorylation Sites
Previous Article in Special Issue
Evolution of Reproductive Life History in Mammals and the Associated Change of Functional Constraints
Article

Altered Expression of DAAM1 and PREP Induced by Cadmium Toxicity Is Counteracted by Melatonin in the Rat Testis

1
Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate “F. Bottazzi”, Università degli Studi della Campania “Luigi Vanvitelli”, via Costantinopoli, 16, 80138 Napoli, Italy
2
Laboratoire LR11ES41 Génétique Biodiversité et Valorisation des Bio-Ressourcés, Institut Supérieur de Biotechnologie de Monastir, Université de Monastir, Rue Taher Haddad, Monastir 5000, Tunisia
3
Department of Cellular and Structural Biology, UT Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Terrence S. Furey
Genes 2021, 12(7), 1016; https://doi.org/10.3390/genes12071016
Received: 3 June 2021 / Revised: 22 June 2021 / Accepted: 29 June 2021 / Published: 30 June 2021
(This article belongs to the Special Issue Male Reproduction: Regulation, Differentiation and Epigenetics)
Cadmium (Cd) is one of the most toxic pollutants for health due to its accumulation in several tissues, including testis. This report confirms that Cd increased oxidative stress and apoptosis of germ and somatic cells and provoked testicular injury, as documented by biomolecular and histological alterations, i.e., CAT and SOD activity, the protein level of steroidogenic enzymes (StAR and 3β-HSD), and morphometric parameters. Additionally, it further documents the melatonin (MLT) coadministration produces affects in mitigating Cd-induced toxicity on adult rat testis, as demonstrated by the reduction of oxidative stress and apoptosis, with reversal of the observed histological changes; moreover, a role of MLT in partially restoring steroidogenic enzymes expression was evidenced. Importantly, the cytoarchitecture of testicular cells was perturbed by Cd exposure, as highlighted by impairment of the expression and localization of two cytoskeleton-associated proteins DAAM1 and PREP, which are involved in the germ cells’ differentiation into spermatozoa, altering the normal spermatogenesis. Here, for the first time, we found that the co-treatment with MLT attenuated the Cd-induced toxicity on the testicular DAAM1 and PREP expression. The combined findings provide additional clues about a protective effect of MLT against Cd-induced testicular toxicity by acting on DAAM1 and PREP expression, encouraging further studies to prove its effectiveness in human health. View Full-Text
Keywords: melatonin; cadmium; testis; oxidative stress; endocrine disrupters; cytoskeleton; DAAM1; PREP melatonin; cadmium; testis; oxidative stress; endocrine disrupters; cytoskeleton; DAAM1; PREP
Show Figures

Graphical abstract

MDPI and ACS Style

Venditti, M.; Ben Rhouma, M.; Romano, M.Z.; Messaoudi, I.; Reiter, R.J.; Minucci, S. Altered Expression of DAAM1 and PREP Induced by Cadmium Toxicity Is Counteracted by Melatonin in the Rat Testis. Genes 2021, 12, 1016. https://doi.org/10.3390/genes12071016

AMA Style

Venditti M, Ben Rhouma M, Romano MZ, Messaoudi I, Reiter RJ, Minucci S. Altered Expression of DAAM1 and PREP Induced by Cadmium Toxicity Is Counteracted by Melatonin in the Rat Testis. Genes. 2021; 12(7):1016. https://doi.org/10.3390/genes12071016

Chicago/Turabian Style

Venditti, Massimo, Mariem Ben Rhouma, Maria Z. Romano, Imed Messaoudi, Russel J. Reiter, and Sergio Minucci. 2021. "Altered Expression of DAAM1 and PREP Induced by Cadmium Toxicity Is Counteracted by Melatonin in the Rat Testis" Genes 12, no. 7: 1016. https://doi.org/10.3390/genes12071016

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop