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Article

Dynamics of Specific cfDNA Fragments in the Plasma of Full Marathon Participants

1
Laboratory of Laboratory-Sports Medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
2
Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
3
Research and Development Division, Blue Industries Inc., ArcaCentral Bldg 14F, 1-1-1 Kinshi, Sumida, Tokyo 130-0013, Japan
4
Tsukuba i-Laboratory LLP, 2-1-17 Amakubo, Tsukuba 305-0005, Ibaraki, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Paweł Cięszczyk
Genes 2021, 12(5), 676; https://doi.org/10.3390/genes12050676
Received: 12 March 2021 / Revised: 15 April 2021 / Accepted: 27 April 2021 / Published: 30 April 2021
(This article belongs to the Section Human Genomics and Genetic Diseases)
Plasma cell-free DNA (cfDNA) is frequently analyzed using liquid biopsy to investigate cancer markers. We hypothesized that this concept might be applicable in exercise physiology. Here, we aimed to identify specific cfDNA (spcfDNA) sequences in the plasma of healthy humans using next-generation sequencing (NGS) and clearly define the dynamics regarding spcfDNA-fragment levels upon extreme exercises, such as running a full marathon. NGS analysis was performed using cfDNA of pooled plasma collected from healthy participants. We confirmed that the TaqMan-qPCR assay had high sensitivity and found that the spcfDNA sequence abundance was 16,600-fold higher than that in a normal genomic region. We then used the TaqMan-qPCR assay to investigate the dynamics of spcfDNA-fragment levels upon running a full marathon. The spcfDNA fragment levels were significantly increased post-marathon. Furthermore, spcfDNA fragment levels were strongly correlated with white blood cell and plasma myoglobin concentrations. These results suggest the spcfDNA fragments identified in this study were highly sensitive as markers of extreme physical stress. The findings of this study may provide new insights into exercise physiology and genome biology in humans. View Full-Text
Keywords: cfDNA; next-generation sequencing; full marathon; exercise; physiology cfDNA; next-generation sequencing; full marathon; exercise; physiology
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MDPI and ACS Style

Sugasawa, T.; Fujita, S.-i.; Kuji, T.; Ishibashi, N.; Tamai, K.; Kawakami, Y.; Takekoshi, K. Dynamics of Specific cfDNA Fragments in the Plasma of Full Marathon Participants. Genes 2021, 12, 676. https://doi.org/10.3390/genes12050676

AMA Style

Sugasawa T, Fujita S-i, Kuji T, Ishibashi N, Tamai K, Kawakami Y, Takekoshi K. Dynamics of Specific cfDNA Fragments in the Plasma of Full Marathon Participants. Genes. 2021; 12(5):676. https://doi.org/10.3390/genes12050676

Chicago/Turabian Style

Sugasawa, Takehito, Shin-ichiro Fujita, Tomoaki Kuji, Noriyo Ishibashi, Kenshirou Tamai, Yasushi Kawakami, and Kazuhiro Takekoshi. 2021. "Dynamics of Specific cfDNA Fragments in the Plasma of Full Marathon Participants" Genes 12, no. 5: 676. https://doi.org/10.3390/genes12050676

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