Next Article in Journal
Genomic Features of Open Chromatin Regions (OCRs) in Wild Soybean and Their Effects on Gene Expressions
Next Article in Special Issue
α-Fodrin in Cytoskeletal Organization and the Activity of Certain Key Microtubule Kinesins
Previous Article in Journal
Deletion of the Imprinted Phlda2 Gene Increases Placental Passive Permeability in the Mouse
 
 
Article

Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies

1
Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland
2
Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, 61-614 Poznan, Poland
3
Institute of Biochemistry and Biophysics Polish Academy of Sciences, 02-106 Warszawa, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Chandrasekhar Natarajan
Genes 2021, 12(5), 638; https://doi.org/10.3390/genes12050638
Received: 1 April 2021 / Revised: 18 April 2021 / Accepted: 20 April 2021 / Published: 25 April 2021
(This article belongs to the Special Issue Proteins: Proteomics and Beyond)
The slime mold Dictyostelium discoideum’s life cycle includes different unicellular and multicellular stages that provide a convenient model for research concerning intracellular and intercellular mechanisms influencing mitochondria’s structure and function. We aim to determine the differences between the mitochondria isolated from the slime mold regarding its early developmental stages induced by starvation, namely the unicellular (U), aggregation (A) and streams (S) stages, at the bioenergetic and proteome levels. We measured the oxygen consumption of intact cells using the Clarke electrode and observed a distinct decrease in mitochondrial coupling capacity for stage S cells and a decrease in mitochondrial coupling efficiency for stage A and S cells. We also found changes in spare respiratory capacity. We performed a wide comparative proteomic study. During the transition from the unicellular stage to the multicellular stage, important proteomic differences occurred in stages A and S relating to the proteins of the main mitochondrial functional groups, showing characteristic tendencies that could be associated with their ongoing adaptation to starvation following cell reprogramming during the switch to gluconeogenesis. We suggest that the main mitochondrial processes are downregulated during the early developmental stages, although this needs to be verified by extending analogous studies to the next slime mold life cycle stages. View Full-Text
Keywords: mitochondrial proteins; mass spectrometry; Dictyostelium discoideum; starvation; coupling efficiency; coupling capacity; spare respiratory capacity; qualitative and quantitative comparative studies mitochondrial proteins; mass spectrometry; Dictyostelium discoideum; starvation; coupling efficiency; coupling capacity; spare respiratory capacity; qualitative and quantitative comparative studies
Show Figures

Figure 1

MDPI and ACS Style

Mazur, M.; Wojciechowska, D.; Sitkiewicz, E.; Malinowska, A.; Świderska, B.; Kmita, H.; Wojtkowska, M. Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies. Genes 2021, 12, 638. https://doi.org/10.3390/genes12050638

AMA Style

Mazur M, Wojciechowska D, Sitkiewicz E, Malinowska A, Świderska B, Kmita H, Wojtkowska M. Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies. Genes. 2021; 12(5):638. https://doi.org/10.3390/genes12050638

Chicago/Turabian Style

Mazur, Monika, Daria Wojciechowska, Ewa Sitkiewicz, Agata Malinowska, Bianka Świderska, Hanna Kmita, and Małgorzata Wojtkowska. 2021. "Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies" Genes 12, no. 5: 638. https://doi.org/10.3390/genes12050638

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop