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Volume 12
Issue 2
10.3390/genes12020286
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Review

Applications of Microsatellite Markers for the Characterization of Olive Genetic Resources of Tunisia

by
Olfa Saddoud Debbabi
1,2,*,
Sameh Rahmani Mnasri
1,2,
Fathi Ben Amar
2,
M’barek Ben Naceur
1,
Cinzia Montemurro
3,* and
Monica Marilena Miazzi
3
1
Banque Nationale de Gènes, Boulevard du Leader Yesser Arafet, Charguia 1, 1080 Tunis, Tunisia
2
Institut de l’Olivier, University of Sfax, Route de l’Aeroport Km 0.5, 3029 Sfax, Tunisia
3
Department of Soil, Plant and Food Sciences (DISPA), University of Bari, Via Amendola 165/A, 70126 Bari, Italy
*
Authors to whom correspondence should be addressed.
Genes 2021, 12(2), 286; https://doi.org/10.3390/genes12020286
Submission received: 26 December 2020 / Revised: 5 February 2021 / Accepted: 9 February 2021 / Published: 18 February 2021
(This article belongs to the Special Issue Research Progress and Application Prospects of Microsatellites)
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Abstract

Among the countries of the Mediterranean Basin, Tunisia is located at the crossroad for the immigration of several civilizations over the last two millennia, becoming a strategic place for gene flow, and a secondary center of diversity for olive species. Olive is one of the principal crop species in Tunisia and now it strongly characterizes the rural landscape of the country. In recent years, collecting missions on farm and in situ were carried out by various institutes, with special emphasis given to ex situ collections serving as a reference for the identification of olive germplasm. Simple Sequence Repeats (SSRs) represent the easiest and cheapest markers for olive genetic fingerprinting and have been the tool of choice for studying the genetic diversity of this crop in Tunisia, to resolve cases of homonymy and synonymy among the commercialized varieties, to identify rare cultivars, to improve knowledge about the genetic variability of this crop, to identify a hot spot of olive biodiversity in the Tunisian oasis of Degache, and to enrich the national reference collection of olive varieties. The present review describes the state of the art of the genetic characterization of the Tunisian olive germplasm and illustrate the progress obtained through the SSR markers, in individuating interesting genotypes that could be used for facing incoming problems determined by climate changes.
Keywords: fingerprinting; genetic diversity; olive; plant genetic resources; SSR fingerprinting; genetic diversity; olive; plant genetic resources; SSR

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MDPI and ACS Style

Saddoud Debbabi, O.; Rahmani Mnasri, S.; Ben Amar, F.; Ben Naceur, M.; Montemurro, C.; Miazzi, M.M. Applications of Microsatellite Markers for the Characterization of Olive Genetic Resources of Tunisia. Genes 2021, 12, 286. https://doi.org/10.3390/genes12020286

AMA Style

Saddoud Debbabi O, Rahmani Mnasri S, Ben Amar F, Ben Naceur M, Montemurro C, Miazzi MM. Applications of Microsatellite Markers for the Characterization of Olive Genetic Resources of Tunisia. Genes. 2021; 12(2):286. https://doi.org/10.3390/genes12020286

Chicago/Turabian Style

Saddoud Debbabi, Olfa, Sameh Rahmani Mnasri, Fathi Ben Amar, M’barek Ben Naceur, Cinzia Montemurro, and Monica Marilena Miazzi. 2021. "Applications of Microsatellite Markers for the Characterization of Olive Genetic Resources of Tunisia" Genes 12, no. 2: 286. https://doi.org/10.3390/genes12020286

APA Style

Saddoud Debbabi, O., Rahmani Mnasri, S., Ben Amar, F., Ben Naceur, M., Montemurro, C., & Miazzi, M. M. (2021). Applications of Microsatellite Markers for the Characterization of Olive Genetic Resources of Tunisia. Genes, 12(2), 286. https://doi.org/10.3390/genes12020286

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