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Article

Leishmania Sexual Reproductive Strategies as Resolved through Computational Methods Designed for Aneuploid Genomes

1
Discovery Bioinformatics, NextCure Inc., Beltsville, MD 20705, USA
2
Department of Molecular Microbiology, School of Medicine, Washington University, St. Louis, MO 63110, USA
3
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20814, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Sam Alsford
Genes 2021, 12(2), 167; https://doi.org/10.3390/genes12020167
Received: 9 November 2020 / Revised: 21 January 2021 / Accepted: 21 January 2021 / Published: 26 January 2021
A cryptic sexual reproductive cycle in Leishmania has been inferred through population genetic studies revealing the presence of hybrid genotypes in natural isolates, with attempts made to decipher sexual strategies by studying complex chromosomal inheritance patterns. A more informative approach is to study the products of controlled, laboratory-based experiments where known strains or species are crossed in the sand fly vector to generate hybrid progeny. These hybrids can be subsequently studied through high resolution sequencing technologies and software suites such as PAINT that disclose inheritance patterns including ploidies, parental chromosome contributions and recombinations, all of which can inform the sexual strategy. In this work, we discuss the computational methods in PAINT that can be used to interpret the sexual strategies adopted specifically by aneuploid organisms and summarize how PAINT has been applied to the analysis of experimental hybrids to reveal meiosis-like sexual recombination in Leishmania. View Full-Text
Keywords: aneuploidy; meiosis; next-generation sequencing; bioinformatics; chromosomal inheritance; Leishmania; clonality and sexuality aneuploidy; meiosis; next-generation sequencing; bioinformatics; chromosomal inheritance; Leishmania; clonality and sexuality
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MDPI and ACS Style

Shaik, J.S.; Dobson, D.E.; Sacks, D.L.; Beverley, S.M. Leishmania Sexual Reproductive Strategies as Resolved through Computational Methods Designed for Aneuploid Genomes. Genes 2021, 12, 167. https://doi.org/10.3390/genes12020167

AMA Style

Shaik JS, Dobson DE, Sacks DL, Beverley SM. Leishmania Sexual Reproductive Strategies as Resolved through Computational Methods Designed for Aneuploid Genomes. Genes. 2021; 12(2):167. https://doi.org/10.3390/genes12020167

Chicago/Turabian Style

Shaik, Jahangheer S., Deborah E. Dobson, David L. Sacks, and Stephen M. Beverley 2021. "Leishmania Sexual Reproductive Strategies as Resolved through Computational Methods Designed for Aneuploid Genomes" Genes 12, no. 2: 167. https://doi.org/10.3390/genes12020167

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