Transcription Factor 21 Promotes Chicken Adipocyte Differentiation at Least in Part via Activating MAPK/JNK Signaling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Experimental Design
2.2. Oil Red O Staining and Extraction
2.3. Western Blotting
2.4. Luciferase Reporter Assay
2.5. RT-qPCR
2.6. Statistical Analysis
3. Results
3.1. Overexpression of TCF21 Leads to Enhanced Lipid Droplets Accumulation
3.2. MAPK/JNK Signaling Pathway Was Activated by TCF21 Overexpression
3.3. MAPK/JNK Signaling and Lipid Droplets Accumulation Are Inhibited by SP600125 in a Dose-Dependent Manner
3.4. Inhibition of MAPK/JNK Signaling Attenuates TCF21-Mediated Promotion of Preadipocyte Differentiation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
TCF21 | transcription factor 21 |
ICP | immortalized chicken preadipocytes cell line |
LV-TCF21 | preadipocyte cell line stably overexpressing TCF21 |
LV-control | control preadipocyte cell line |
JNK | c-Jun N-terminal kinases |
PPARγ | peroxisome proliferator-activated receptor γ |
C/EBPα | CCAAT/enhancer binding protein α |
A-FABP | adipocyte fatty acid binding protein |
LPL | lipoprotein lipase |
TBP | TATA-box binding protein |
ERK | extracellular signal-regulated kinase |
NLK | nemo-like kinase |
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Gene | Accession Number | Primer Sequence (5’ to 3’) | Product Length (bp) |
---|---|---|---|
PPARγ | NM_001001460 | F:GTGCAATCAAAATGGAGCC | 170 |
R:CTTACAACCTTCACATGCAT | |||
C/EBPa | NM_001031459 | F:GCGACATCTGCGAGAACG | 266 |
R:GTACAGCGGGTCGAGCTT | |||
A-FABP | NM_204290 | F:ATGTGCGACCAGTTTGT R:TCACCATTGATGCTGATAG | 143 |
LPL | NM_205282 | F:ATGTTCATTGATTGGATGGAGGAG | 159 |
R:AAAGGTGGGACCAGCAGGAT | |||
TBP | NM_205103 | F:GCGTTTTGCTGCTGTTATTATGAG | 122 |
R:TCCTTGCTGCCAGTCTGGAC |
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Zhang, X.; Cheng, B.; Jiang, H.; Liu, C.; Cao, Z.; Luan, P.; Wang, N.; Li, H. Transcription Factor 21 Promotes Chicken Adipocyte Differentiation at Least in Part via Activating MAPK/JNK Signaling. Genes 2021, 12, 1971. https://doi.org/10.3390/genes12121971
Zhang X, Cheng B, Jiang H, Liu C, Cao Z, Luan P, Wang N, Li H. Transcription Factor 21 Promotes Chicken Adipocyte Differentiation at Least in Part via Activating MAPK/JNK Signaling. Genes. 2021; 12(12):1971. https://doi.org/10.3390/genes12121971
Chicago/Turabian StyleZhang, Xinyang, Bohan Cheng, Haixu Jiang, Chang Liu, Zhiping Cao, Peng Luan, Ning Wang, and Hui Li. 2021. "Transcription Factor 21 Promotes Chicken Adipocyte Differentiation at Least in Part via Activating MAPK/JNK Signaling" Genes 12, no. 12: 1971. https://doi.org/10.3390/genes12121971