Defining the Influence of the A12.2 Subunit on Transcription Elongation and Termination by RNA Polymerase I In Vivo
Abstract
:1. Introduction
2. Materials and Methods
2.1. Native Elongating Transcript Sequencing (NET-seq) Library Preparation and Sequencing
2.2. NET-seq Data Formatting and Analysis
3. Results
3.1. WT and rpa12Δ NET-seq Libraries Are Highly Reproducible
3.2. RPA12 Deletion Alters Pol I Occupancy throughout the 35S Gene
3.3. Changes in Pol I Occupancy Are Template Sequence Dependent
3.4. Deletion of RPA12 Results in Pol I Occupancy Downstream of T1 and T2
4. Discussion
4.1. Pol I Occupancy Patterns within the 35S Gene Change in Response to RPA12 Deletion
4.2. S. cerevisiae Contains a Putative Third Pol I Terminator Region
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Clarke, A.M.; Huffines, A.K.; Edwards, Y.J.K.; Petit, C.M.; Schneider, D.A. Defining the Influence of the A12.2 Subunit on Transcription Elongation and Termination by RNA Polymerase I In Vivo. Genes 2021, 12, 1939. https://doi.org/10.3390/genes12121939
Clarke AM, Huffines AK, Edwards YJK, Petit CM, Schneider DA. Defining the Influence of the A12.2 Subunit on Transcription Elongation and Termination by RNA Polymerase I In Vivo. Genes. 2021; 12(12):1939. https://doi.org/10.3390/genes12121939
Chicago/Turabian StyleClarke, Andrew M., Abigail K. Huffines, Yvonne J. K. Edwards, Chad M. Petit, and David A. Schneider. 2021. "Defining the Influence of the A12.2 Subunit on Transcription Elongation and Termination by RNA Polymerase I In Vivo" Genes 12, no. 12: 1939. https://doi.org/10.3390/genes12121939