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Article

Associations of Polymorphisms Localized in the 3′UTR Regions of the KRAS, NRAS, MAPK1 Genes with Laryngeal Squamous Cell Carcinoma

1
Institute of Biology Systems and Genetic Research, Lithuanian University of Health Sciences, 50103 Kaunas, Lithuania
2
Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 50161 Kaunas, Lithuania
*
Author to whom correspondence should be addressed.
Academic Editors: Michele Cioffi and Maria Teresa Vietri
Genes 2021, 12(11), 1679; https://doi.org/10.3390/genes12111679
Received: 14 September 2021 / Revised: 18 October 2021 / Accepted: 21 October 2021 / Published: 23 October 2021
(This article belongs to the Special Issue Genotype-Phenotype Study in Disease)
Background: Genetic variations, localized in the 3′ untranslated region (UTR) in mitogen-activated protein kinase (MAPK) pathway-related genes, may alter the transcription and impact the pathogenesis of laryngeal squamous cell carcinoma (LSCC). The present study investigated the associations of single-nucleotide polymorphisms (SNP), localized in the 3′UTR) of the KRAS, NRAS, and MAPK1 genes with LSCC risk and clinicopathological features. Methods: Genomic DNA and clinical data were collected from 327 adult men with LSCC. The control group was formed from 333 healthy men. Genotyping of the SNPs was performed using TaqMan SNP genotyping assays. Five KRAS, NRAS, and MAPK1 polymorphisms were analyzed. All studied genotypes were in Hardy–Weinberg equilibrium and had the same allele distribution as the 1000 Genomes project Phase 3 dataset for the European population. Results: Significant associations of the studied SNPs with reduced LSCC risk were observed between NRAS rs14804 major genotype CC. Significant associations of the studied SNPs with clinicopathologic variables were also observed between NRAS rs14804 minor T allele and advanced tumor stage and positive lymph node status. SNP of MAPK1 rs9340 was associated with distant metastasis. Moreover, haplotype analysis of two KRAS SNPs rs712 and rs7973450 revealed that TG haplotype was associated with positive lymph node status in LSCC patients. Conclusions: According to the present study, 3′UTR SNP in the NRAS and MAPK1 genes may contribute to the identifications of patients at higher risk of LSCC lymph node and distant metastasis development. View Full-Text
Keywords: laryngeal squamous cell carcinoma; single-nucleotide polymorphisms; mitogen-activated protein kinases; KRAS; NRAS; MAPK1 laryngeal squamous cell carcinoma; single-nucleotide polymorphisms; mitogen-activated protein kinases; KRAS; NRAS; MAPK1
MDPI and ACS Style

Insodaite, R.; Smalinskiene, A.; Liutkevicius, V.; Ulozas, V.; Poceviciute, R.; Bielevicius, A.; Kucinskas, L. Associations of Polymorphisms Localized in the 3′UTR Regions of the KRAS, NRAS, MAPK1 Genes with Laryngeal Squamous Cell Carcinoma. Genes 2021, 12, 1679. https://doi.org/10.3390/genes12111679

AMA Style

Insodaite R, Smalinskiene A, Liutkevicius V, Ulozas V, Poceviciute R, Bielevicius A, Kucinskas L. Associations of Polymorphisms Localized in the 3′UTR Regions of the KRAS, NRAS, MAPK1 Genes with Laryngeal Squamous Cell Carcinoma. Genes. 2021; 12(11):1679. https://doi.org/10.3390/genes12111679

Chicago/Turabian Style

Insodaite, Ruta, Alina Smalinskiene, Vykintas Liutkevicius, Virgilijus Ulozas, Roberta Poceviciute, Arunas Bielevicius, and Laimutis Kucinskas. 2021. "Associations of Polymorphisms Localized in the 3′UTR Regions of the KRAS, NRAS, MAPK1 Genes with Laryngeal Squamous Cell Carcinoma" Genes 12, no. 11: 1679. https://doi.org/10.3390/genes12111679

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