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Home and Away: The Role of Non-Coding RNA in Intracellular and Intercellular DNA Damage Response
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DNA Damage Response and Cell Cycle Regulation in Pluripotent Stem Cells

1
Department of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Hong Kong, China
2
Shenzhen Key Laboratory of Fertility Regulation, Reproductive Medicine Center, The University of Hong Kong Shenzhen Hospital, Shenzhen 518009, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Jean Molinier
Genes 2021, 12(10), 1548; https://doi.org/10.3390/genes12101548
Received: 6 September 2021 / Revised: 27 September 2021 / Accepted: 28 September 2021 / Published: 29 September 2021
(This article belongs to the Special Issue Reciprocal Links between RNA Metabolism and DNA Damage)
Pluripotent stem cells (PSCs) hold great promise in cell-based therapy because of their pluripotent property and the ability to proliferate indefinitely. Embryonic stem cells (ESCs) derived from inner cell mass (ICM) possess unique cell cycle control with shortened G1 phase. In addition, ESCs have high expression of homologous recombination (HR)-related proteins, which repair double-strand breaks (DSBs) through HR or the non-homologous end joining (NHEJ) pathway. On the other hand, the generation of induced pluripotent stem cells (iPSCs) by forced expression of transcription factors (Oct4, Sox2, Klf4, c-Myc) is accompanied by oxidative stress and DNA damage. The DNA repair mechanism of DSBs is therefore critical in determining the genomic stability and efficiency of iPSCs generation. Maintaining genomic stability in PSCs plays a pivotal role in the proliferation and pluripotency of PSCs. In terms of therapeutic application, genomic stability is the key to reducing the risks of cancer development due to abnormal cell replication. Over the years, we and other groups have identified important regulators of DNA damage response in PSCs, including FOXM1, SIRT1 and PUMA. They function through transcription regulation of downstream targets (P53, CDK1) that are involved in cell cycle regulations. Here, we review the fundamental links between the PSC-specific HR process and DNA damage response, with a focus on the roles of FOXM1 and SIRT1 on maintaining genomic integrity. View Full-Text
Keywords: DNA damage; cell cycle regulation; pluripotent stem cells (PSCs); FOXM1; SIRT1 DNA damage; cell cycle regulation; pluripotent stem cells (PSCs); FOXM1; SIRT1
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MDPI and ACS Style

Chen, A.C.H.; Peng, Q.; Fong, S.W.; Lee, K.C.; Yeung, W.S.B.; Lee, Y.L. DNA Damage Response and Cell Cycle Regulation in Pluripotent Stem Cells. Genes 2021, 12, 1548. https://doi.org/10.3390/genes12101548

AMA Style

Chen ACH, Peng Q, Fong SW, Lee KC, Yeung WSB, Lee YL. DNA Damage Response and Cell Cycle Regulation in Pluripotent Stem Cells. Genes. 2021; 12(10):1548. https://doi.org/10.3390/genes12101548

Chicago/Turabian Style

Chen, Andy C.H., Qian Peng, Sze W. Fong, Kai C. Lee, William S.B. Yeung, and Yin L. Lee. 2021. "DNA Damage Response and Cell Cycle Regulation in Pluripotent Stem Cells" Genes 12, no. 10: 1548. https://doi.org/10.3390/genes12101548

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