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Open AccessArticle

Long-Term Impact of Suppressive Antibiotic Therapy on Intestinal Microbiota

1
Servicio de Enfermedades Infecciosas, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), and Red Española de Investigación en Patología Infecciosa (REIPI), 28034 Madrid, Spain
2
Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), and Red Española de Investigación en Patología Infecciosa (REIPI), 28034 Madrid, Spain
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Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, Spain
*
Author to whom correspondence should be addressed.
Genes 2021, 12(1), 41; https://doi.org/10.3390/genes12010041
Received: 27 November 2020 / Revised: 20 December 2020 / Accepted: 29 December 2020 / Published: 30 December 2020
(This article belongs to the Special Issue Genetics of Antimicrobial Resistance)
The aim was to describe the safety of indefinite administration of antibiotics, the so-called suppressive antibiotic therapy (SAT) and to provide insight into their impact on gut microbiota. 17 patients with SAT were recruited, providing a fecal sample. Bacterial composition was determined by 16S rDNA massive sequencing, and their viability was explored by PCR-DGGE with and without propidium monoazide. Presence of antibiotic multirresistant bacteria was explored through the culture of feces in selective media. High intra-individual variability in the genera distribution regardless of the antibiotic or antibiotic administration ingestion period, with few statistically significant differences detected by Bray-Curtis distance-based principle component analysis, permutational multivariate analysis of variance and linear discriminant analysis effect size analysis. However, the microbiota composition of patients treated with both beta-lactams and sulfonamides clustered by a heat map. Curiously, the detection of antibiotic resistant bacteria was almost anecdotic and CTX-M-15-producing E. coli were detected in two subjects. Our work demonstrates the overall clinical safety of SAT and the low rate of the selection of multidrug-resistant bacteria triggered by this therapy. We also describe the composition of intestinal microbiota under the indefinite use of antibiotics for the first time. View Full-Text
Keywords: suppressive antibiotic therapy; gut microbiota; PCR-DGGE; bacterial viability; propidium monoazide; antibiotic multirresistant colonization suppressive antibiotic therapy; gut microbiota; PCR-DGGE; bacterial viability; propidium monoazide; antibiotic multirresistant colonization
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MDPI and ACS Style

Escudero-Sánchez, R.; Ponce-Alonso, M.; Barragán-Prada, H.; Morosini, M.I.; Cantón, R.; Cobo, J.; del Campo, R. Long-Term Impact of Suppressive Antibiotic Therapy on Intestinal Microbiota. Genes 2021, 12, 41.

AMA Style

Escudero-Sánchez R, Ponce-Alonso M, Barragán-Prada H, Morosini MI, Cantón R, Cobo J, del Campo R. Long-Term Impact of Suppressive Antibiotic Therapy on Intestinal Microbiota. Genes. 2021; 12(1):41.

Chicago/Turabian Style

Escudero-Sánchez, Rosa; Ponce-Alonso, Manuel; Barragán-Prada, Hugo; Morosini, María I.; Cantón, Rafael; Cobo, Javier; del Campo, Rosa. 2021. "Long-Term Impact of Suppressive Antibiotic Therapy on Intestinal Microbiota" Genes 12, no. 1: 41.

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