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Open AccessArticle

Newly Emerged Serotype 1c of Shigella flexneri: Multiple Origins and Changing Drug Resistance Landscape

1
Division of Biomedical Science and Biochemistry, Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia
2
Research School of Computer Science & Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia
*
Author to whom correspondence should be addressed.
Genes 2020, 11(9), 1042; https://doi.org/10.3390/genes11091042
Received: 28 July 2020 / Revised: 31 August 2020 / Accepted: 1 September 2020 / Published: 3 September 2020
(This article belongs to the Section Microbial Genetics and Genomics)
Bacillary dysentery caused by Shigella flexneri is a major cause of under-five mortality in developing countries, where a novel S. flexneri serotype 1c has become very common since the 1980s. However, the origin and diversification of serotype 1c remain poorly understood. To understand the evolution of serotype 1c and their antimicrobial resistance, we sequenced and analyzed the whole-genome of 85 clinical isolates from the United Kingdom, Egypt, Bangladesh, Vietnam, and Japan belonging to serotype 1c and related serotypes of 1a, 1b and Y/Yv. We identified up to three distinct O-antigen modifying genes in S. flexneri 1c strains, which were acquired from three different bacteriophages. Our analysis shows that S. flexneri 1c strains have originated from serotype 1a and serotype 1b strains after the acquisition of bacteriophage-encoding gtrIc operon. The maximum-likelihood phylogenetic analysis using core genes suggests two distinct S. flexneri 1c lineages, one specific to Bangladesh, which originated from ancestral serotype 1a strains and the other from the United Kingdom, Egypt, and Vietnam originated from ancestral serotype 1b strains. We also identified 63 isolates containing multiple drug-resistant genes in them conferring resistance against streptomycin, sulfonamide, quinolone, trimethoprim, tetracycline, chloramphenicol, and beta-lactamase. Furthermore, antibiotic susceptibility assays showed 83 (97.6%) isolates as either complete or intermediate resistance to the WHO-recommended first- and second-line drugs. This changing drug resistance pattern demonstrates the urgent need for drug resistance surveillance and renewed treatment guidelines. View Full-Text
Keywords: Shigella flexneri serotype 1c; bacteriophage; serotype evolution; multidrug resistance Shigella flexneri serotype 1c; bacteriophage; serotype evolution; multidrug resistance
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MDPI and ACS Style

Parajuli, P.; Minh, B.Q.; Verma, N.K. Newly Emerged Serotype 1c of Shigella flexneri: Multiple Origins and Changing Drug Resistance Landscape. Genes 2020, 11, 1042. https://doi.org/10.3390/genes11091042

AMA Style

Parajuli P, Minh BQ, Verma NK. Newly Emerged Serotype 1c of Shigella flexneri: Multiple Origins and Changing Drug Resistance Landscape. Genes. 2020; 11(9):1042. https://doi.org/10.3390/genes11091042

Chicago/Turabian Style

Parajuli, Pawan; Minh, Bui Q.; Verma, Naresh K. 2020. "Newly Emerged Serotype 1c of Shigella flexneri: Multiple Origins and Changing Drug Resistance Landscape" Genes 11, no. 9: 1042. https://doi.org/10.3390/genes11091042

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