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Article

Transcriptomic Analysis of Streptococcus suis in Response to Ferrous Iron and Cobalt Toxicity

by 1,2, 1,2, 1,2 and 1,2,*
1
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
2
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(9), 1035; https://doi.org/10.3390/genes11091035
Received: 16 August 2020 / Revised: 29 August 2020 / Accepted: 1 September 2020 / Published: 2 September 2020
(This article belongs to the Section Microbial Genetics and Genomics)
Streptococcus suis is a zoonotic pathogen causing serious infections in swine and humans. Although metals are essential for life, excess amounts of metals are toxic to bacteria. Transcriptome-level data of the mechanisms for resistance to metal toxicity in S. suis are available for no metals other than zinc. Herein, we explored the transcriptome-level changes in S. suis in response to ferrous iron and cobalt toxicity by RNA sequencing. Many genes were differentially expressed in the presence of excess ferrous iron and cobalt. Most genes in response to cobalt toxicity showed the same expression trends as those in response to ferrous iron toxicity. qRT-PCR analysis of the selected genes confirmed the accuracy of RNA sequencing results. Bioinformatic analysis of the differentially expressed genes indicated that ferrous iron and cobalt have similar effects on the cellular processes of S. suis. Ferrous iron treatment resulted in down-regulation of several oxidative stress tolerance-related genes and up-regulation of the genes in an amino acid ABC transporter operon. Expression of several genes in the arginine deiminase system was down-regulated after ferrous iron and cobalt treatment. Collectively, our results suggested that S. suis alters the expression of multiple genes to respond to ferrous iron and cobalt toxicity. View Full-Text
Keywords: Streptococcus suis; transcriptome; ferrous iron; cobalt; RNA sequencing Streptococcus suis; transcriptome; ferrous iron; cobalt; RNA sequencing
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MDPI and ACS Style

Jia, M.; Wei, M.; Zhang, Y.; Zheng, C. Transcriptomic Analysis of Streptococcus suis in Response to Ferrous Iron and Cobalt Toxicity. Genes 2020, 11, 1035. https://doi.org/10.3390/genes11091035

AMA Style

Jia M, Wei M, Zhang Y, Zheng C. Transcriptomic Analysis of Streptococcus suis in Response to Ferrous Iron and Cobalt Toxicity. Genes. 2020; 11(9):1035. https://doi.org/10.3390/genes11091035

Chicago/Turabian Style

Jia, Mengdie, Man Wei, Yunzeng Zhang, and Chengkun Zheng. 2020. "Transcriptomic Analysis of Streptococcus suis in Response to Ferrous Iron and Cobalt Toxicity" Genes 11, no. 9: 1035. https://doi.org/10.3390/genes11091035

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