Next Article in Journal
Comprehensive Genomic Analysis Reveals the Prognostic Role of LRRK2 Copy-Number Variations in Human Malignancies
Next Article in Special Issue
Sexual Difference in the Optimum Environmental Conditions for Growth and Maturation of the Brown Alga Undaria pinnatifida in the Gametophyte Stage
Previous Article in Journal
AML with Myelodysplasia-Related Changes: Development, Challenges, and Treatment Advances
Previous Article in Special Issue
Evidence for Dosage Compensation in Coccinia grandis, a Plant with a Highly Heteromorphic XY System
Open AccessArticle

Sequencing and Analysis of the Sex Determination Region of Populus trichocarpa

Department of Biology, West Virginia University, Morgantown, WV 26506-6057, USA
HudsonAlpha Institute of Biotechnology, 601 Genome Way Northwest, Huntsville, AL 35806, USA
Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
Center for Bioenergy Innovation, Biosciences Division, Oak Ridge National Lab, Oak Ridge, TN 37830, USA
Author to whom correspondence should be addressed.
Genes 2020, 11(8), 843;
Received: 1 July 2020 / Revised: 17 July 2020 / Accepted: 22 July 2020 / Published: 24 July 2020
The ages and sizes of a sex-determination region (SDR) are difficult to determine in non-model species. Due to the lack of recombination and enrichment of repetitive elements in SDRs, the quality of assembly with short sequencing reads is universally low. Unique features present in the SDRs help provide clues about how SDRs are established and how they evolve in the absence of recombination. Several Populus species have been reported with a male heterogametic configuration of sex (XX/XY system) mapped on chromosome 19, but the exact location of the SDR has been inconsistent among species, and thus far, none of these SDRs has been fully assembled in a genomic context. Here we identify the Y-SDR from a Y-linked contig directly from a long-read PacBio assembly of a Populus trichocarpa male individual. We also identified homologous gene sequences in the SDR of P. trichocarpa and the SDR of the W chromosome in Salix purpurea. We show that inverted repeats (IRs) found in the Y-SDR and the W-SDR are lineage-specific. We hypothesize that, although the two IRs are derived from the same orthologous gene within each species, they likely have independent evolutionary histories. Furthermore, the truncated inverted repeats in P. trichocarpa may code for small RNAs that target the homologous gene for RNA-directed DNA methylation. These findings support the hypothesis that diverse sex-determining systems may be achieved through similar evolutionary pathways, thereby providing a possible mechanism to explain the lability of sex-determination systems in plants in general. View Full-Text
Keywords: sex; Populus; genome; inverted repeats; cytokinin response regulator sex; Populus; genome; inverted repeats; cytokinin response regulator
Show Figures

Figure 1

MDPI and ACS Style

Zhou, R.; Macaya-Sanz, D.; Schmutz, J.; Jenkins, J.W.; Tuskan, G.A.; DiFazio, S.P. Sequencing and Analysis of the Sex Determination Region of Populus trichocarpa. Genes 2020, 11, 843.

AMA Style

Zhou R, Macaya-Sanz D, Schmutz J, Jenkins JW, Tuskan GA, DiFazio SP. Sequencing and Analysis of the Sex Determination Region of Populus trichocarpa. Genes. 2020; 11(8):843.

Chicago/Turabian Style

Zhou, Ran; Macaya-Sanz, David; Schmutz, Jeremy; Jenkins, Jerry W.; Tuskan, Gerald A.; DiFazio, Stephen P. 2020. "Sequencing and Analysis of the Sex Determination Region of Populus trichocarpa" Genes 11, no. 8: 843.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop