P14^ARF: The Absence that Makes the Difference

P14^ARF is a tumor suppressor encoded by the CDKN2a locus that is frequently inactivated in human tumors. P14^ARF protein quenches oncogene stimuli by inhibiting cell cycle progression and inducing apoptosis. P14^ARF functions can be played through interactions with several proteins. However, the majority of its activities are notoriously mediated by the p53 protein. Interestingly, recent studies suggest a new role of p14^ARF in the maintenance of chromosome stability. Here, we deepened this new facet of p14^ARF which we believe is relevant to its tumor suppressive role in the cell. To this aim, we generated a monoclonal HCT116 cell line expressing the p14^ARF cDNA cloned in the piggyback vector and then induced aneuploidy by treating HCT116 cells with the CENP-E inhibitor GSK923295. P14^ARF ectopic re-expression restored the near-diploid phenotype of HCT116 cells, confirming that p14^ARF counteracts aneuploid cell generation/proliferation.