Next Article in Journal
Differential Alternative Splicing Genes and Isoform Regulation Networks of Rapeseed (Brassica napus L.) Infected with Sclerotinia sclerotiorum
Next Article in Special Issue
Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae
Previous Article in Journal
Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)
Previous Article in Special Issue
Utility of Common Marmoset (Callithrix jacchus) Embryonic Stem Cells in Liver Disease Modeling, Tissue Engineering and Drug Metabolism
Article

Satellitome Analysis in the Ladybird Beetle Hippodamia variegata (Coleoptera, Coccinellidae)

1
Department of Experimental Biology, Genetic Area, University of Jaén, 23071 Jaén, Spain
2
Department of Organismal Biology, Systematic Biology, Evolutionary Biology Centre, Uppsala University, SE-752 36 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Genes 2020, 11(7), 783; https://doi.org/10.3390/genes11070783
Received: 18 June 2020 / Revised: 9 July 2020 / Accepted: 9 July 2020 / Published: 13 July 2020
(This article belongs to the Special Issue Genes at Ten)
Hippodamia variegata is one of the most commercialized ladybirds used for the biological control of aphid pest species in many economically important crops. This species is the first Coccinellidae whose satellitome has been studied by applying new sequencing technologies and bioinformatics tools. We found that 47% of the H. variegata genome is composed of repeated sequences. We identified 30 satellite DNA (satDNA) families with a median intragenomic divergence of 5.75% and A+T content between 45.6% and 74.7%. This species shows satDNA families with highly variable sizes although the most common size is 100–200 bp. However, we highlight the existence of a satDNA family with a repeat unit of 2 kb, the largest repeat unit described in Coleoptera. PCR amplifications for fluorescence in situ hybridization (FISH) probe generation were performed for the four most abundant satDNA families. FISH with the most abundant satDNA family as a probe shows its pericentromeric location on all chromosomes. This location is coincident with the heterochromatin revealed by C-banding and DAPI staining, also analyzed in this work. Hybridization signals for other satDNA families were located only on certain bivalents and the X chromosome. These satDNAs could be very useful as chromosomal markers due to their reduced location. View Full-Text
Keywords: Coleoptera; Coccinellidae; Hippodamia variegata; ladybird beetle; RepeatExplorer; satellite DNA; satellitome; in situ hybridization Coleoptera; Coccinellidae; Hippodamia variegata; ladybird beetle; RepeatExplorer; satellite DNA; satellitome; in situ hybridization
Show Figures

Figure 1

MDPI and ACS Style

Mora, P.; Vela, J.; Ruiz-Ruano, F.J.; Ruiz-Mena, A.; Montiel, E.E.; Palomeque, T.; Lorite, P. Satellitome Analysis in the Ladybird Beetle Hippodamia variegata (Coleoptera, Coccinellidae). Genes 2020, 11, 783. https://doi.org/10.3390/genes11070783

AMA Style

Mora P, Vela J, Ruiz-Ruano FJ, Ruiz-Mena A, Montiel EE, Palomeque T, Lorite P. Satellitome Analysis in the Ladybird Beetle Hippodamia variegata (Coleoptera, Coccinellidae). Genes. 2020; 11(7):783. https://doi.org/10.3390/genes11070783

Chicago/Turabian Style

Mora, Pablo, Jesús Vela, Francisco J. Ruiz-Ruano, Areli Ruiz-Mena, Eugenia E. Montiel, Teresa Palomeque, and Pedro Lorite. 2020. "Satellitome Analysis in the Ladybird Beetle Hippodamia variegata (Coleoptera, Coccinellidae)" Genes 11, no. 7: 783. https://doi.org/10.3390/genes11070783

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop