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Open AccessArticle

Cellular Environment Remodels the Genomic Fabrics of Functional Pathways in Astrocytes

1
Personalized Genomics Laboratory, Center for Computational Systems Biology, RG Perry College of Engineering, Prairie View A&M University, Prairie View, TX 77446, USA
2
DP Purpura Department of Neuroscience, Albert Einstein College of Medicine, New York, NY 10461, USA
3
Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
4
Department of Biomedical Sciences, College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY 11568, USA
5
Department of Medicine, Albert Einstein College of Medicine, New York, NY 10461, USA
*
Author to whom correspondence should be addressed.
Genes 2020, 11(5), 520; https://doi.org/10.3390/genes11050520
Received: 3 April 2020 / Revised: 27 April 2020 / Accepted: 4 May 2020 / Published: 7 May 2020
(This article belongs to the Special Issue Genomic Fabric Remodeling in Neurological Disorders)
We profiled the transcriptomes of primary mouse cortical astrocytes cultured alone or co-cultured with immortalized precursor oligodendrocytes (Oli-neu cells). Filters between the cell types prevented formation of hetero-cellular gap junction channels but allowed for free exchange of the two culture media. We previously reported that major functional pathways in the Oli-neu cells are remodeled by the proximity of non-touching astrocytes and that astrocytes and oligodendrocytes form a panglial transcriptomic syncytium in the brain. Here, we present evidence that the astrocyte transcriptome likewise changes significantly in the proximity of non-touching Oli-neu cells. Our results indicate that the cellular environment strongly modulates the transcriptome of each cell type and that integration in a heterocellular tissue changes not only the expression profile but also the expression control and networking of the genes in each cell phenotype. The significant decrease of the overall transcription control suggests that in the co-culture astrocytes are closer to their normal conditions from the brain. The Oli-neu secretome regulates astrocyte genes known to modulate neuronal synaptic transmission and remodels calcium, chemokine, NOD-like receptor, PI3K-Akt, and thyroid hormone signaling, as well as actin-cytoskeleton, autophagy, cell cycle, and circadian rhythm pathways. Moreover, the co-culture significantly changes the gene hierarchy in the astrocytes. View Full-Text
Keywords: calcium signaling; chemokine signaling; Cx43; gap junction; NOD-like receptor signaling; oli-neu cells; pannexin1; PI3K-Akt pathway; thyroid hormone pathway calcium signaling; chemokine signaling; Cx43; gap junction; NOD-like receptor signaling; oli-neu cells; pannexin1; PI3K-Akt pathway; thyroid hormone pathway
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MDPI and ACS Style

Iacobas, D.A; Iacobas, S.; Stout, R.F; Spray, D.C. Cellular Environment Remodels the Genomic Fabrics of Functional Pathways in Astrocytes. Genes 2020, 11, 520. https://doi.org/10.3390/genes11050520

AMA Style

Iacobas DA, Iacobas S, Stout RF, Spray DC. Cellular Environment Remodels the Genomic Fabrics of Functional Pathways in Astrocytes. Genes. 2020; 11(5):520. https://doi.org/10.3390/genes11050520

Chicago/Turabian Style

Iacobas, Dumitru A; Iacobas, Sanda; Stout, Randy F; Spray, David C 2020. "Cellular Environment Remodels the Genomic Fabrics of Functional Pathways in Astrocytes" Genes 11, no. 5: 520. https://doi.org/10.3390/genes11050520

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