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AaCOI1, Encoding a CORONATINE INSENSITIVE 1-Like Protein of Artemisia annua L., Is Involved in Development, Defense, and Anthocyanin Synthesis

by 1,2, 1, 1,*, 2, 3 and 3
1
Key Laboratory of Plant Development and Environment Adaption, School of Life Sciences, Shandong University, Qingdao 266237, China
2
Key Laboratory of Education, Department of Hunan Province on Plant Genetics and Molecular Biology, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
3
Institute of Agro-Environment and Ecology, Hunan Academy of Agricultural Sciences, Changsha 410125, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(2), 221; https://doi.org/10.3390/genes11020221
Received: 12 January 2020 / Revised: 11 February 2020 / Accepted: 17 February 2020 / Published: 19 February 2020
(This article belongs to the Section Plant Genetics and Genomics)
Artemisia annua is an important medicinal plant producing the majority of the antimalarial compound artemisinin. Jasmonates are potent inducers of artemisinin accumulation in Artemisisa annua plants. As the receptor of jasmonates, the F-box protein COI1 is critical to the JA signaling required for plant development, defense, and metabolic homeostasis. AaCOI1 from Artemisia annua, homologous to Arabidopsis AtCOI1, encodes a F-box protein located in the nuclei. Expressional profiles of the AaCOI1 in the root, stem, leaves, and inflorescence was investigated. The mRNA abundance of AaCOI1 was the highest in inflorescence, followed by in the leaves. Upon mechanical wounding or MeJA treatment, expression of AaCOI1 was upregulated after 6 h. When ectopically expressed, driven by the native promoter from Arabidopsis thaliana, AaCOI1 could partially complement the JA sensitivity and defense responses, but fully complemented the fertility, and the JA-induced anthocyanin accumulation in a coi1-16 loss-of-function mutant. Our study identifies the paralog of AtCOI1 in Artemisia annua, and revealed its implications in development, hormone signaling, defense, and metabolism. The results provide insight into JA perception in Artemisia annua, and pave the way for novel molecular breeding strategies in the canonical herbs to manipulate the anabolism of pharmaceutic compounds on the phytohormonal level. View Full-Text
Keywords: Artemisia annua; COI1; jasmonate signaling; development; defense; anthocyanin Artemisia annua; COI1; jasmonate signaling; development; defense; anthocyanin
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MDPI and ACS Style

Liu, R.; Wang, J.; Xiao, M.; Gao, X.; Chen, J.; Dai, Y. AaCOI1, Encoding a CORONATINE INSENSITIVE 1-Like Protein of Artemisia annua L., Is Involved in Development, Defense, and Anthocyanin Synthesis. Genes 2020, 11, 221. https://doi.org/10.3390/genes11020221

AMA Style

Liu R, Wang J, Xiao M, Gao X, Chen J, Dai Y. AaCOI1, Encoding a CORONATINE INSENSITIVE 1-Like Protein of Artemisia annua L., Is Involved in Development, Defense, and Anthocyanin Synthesis. Genes. 2020; 11(2):221. https://doi.org/10.3390/genes11020221

Chicago/Turabian Style

Liu, Rong, Jinbiao Wang, Mu Xiao, Xiewang Gao, Jin Chen, and Yanjiao Dai. 2020. "AaCOI1, Encoding a CORONATINE INSENSITIVE 1-Like Protein of Artemisia annua L., Is Involved in Development, Defense, and Anthocyanin Synthesis" Genes 11, no. 2: 221. https://doi.org/10.3390/genes11020221

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