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Current and Emerging Methods for the Synthesis of Single-Stranded DNA

by Min Hao 1,2,3, Jianjun Qiao 1,2,3 and Hao Qi 1,2,3,*
1
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2
Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China
3
SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(2), 116; https://doi.org/10.3390/genes11020116 (registering DOI)
Received: 7 January 2020 / Revised: 16 January 2020 / Accepted: 18 January 2020 / Published: 21 January 2020
(This article belongs to the Section Molecular Genetics and Genomics)
Methods for synthesizing arbitrary single-strand DNA (ssDNA) fragments are rapidly becoming fundamental tools for gene editing, DNA origami, DNA storage, and other applications. To meet the rising application requirements, numerous methods have been developed to produce ssDNA. Some approaches allow the synthesis of freely chosen user-defined ssDNA sequences to overcome the restrictions and limitations of different length, purity, and yield. In this perspective, we provide an overview of the representative ssDNA production strategies and their most significant challenges to enable the readers to make informed choices of synthesis methods and enhance the availability of increasingly inexpensive synthetic ssDNA. We also aim to stimulate a broader interest in the continued development of efficient ssDNA synthesis techniques and improve their applications in future research. View Full-Text
Keywords: ssDNA; chemical synthesis; enzyme synthesis; bacterial-based synthesis ssDNA; chemical synthesis; enzyme synthesis; bacterial-based synthesis
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MDPI and ACS Style

Hao, M.; Qiao, J.; Qi, H. Current and Emerging Methods for the Synthesis of Single-Stranded DNA. Genes 2020, 11, 116.

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