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Article

Comparative Genomics and Functional Studies of Wheat BED-NLR Loci

1
John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
2
Plant Genome and Systems Biology, Helmholtz Center Munich, D-85764 Neuherberg, Germany
*
Author to whom correspondence should be addressed.
A complete list of the 10+ Wheat Genome Project is provided in Table S17, http://www.10wheatgenomes.com/
Genes 2020, 11(12), 1406; https://doi.org/10.3390/genes11121406
Received: 13 February 2020 / Revised: 30 April 2020 / Accepted: 10 May 2020 / Published: 26 November 2020
(This article belongs to the Special Issue NLR Gene Evolution in Plants)
Nucleotide-binding leucine-rich-repeat (LRR) receptors (NLRs) with non-canonical integrated domains (NLR-IDs) are widespread in plant genomes. Zinc-finger BED (named after the Drosophila proteins Boundary Element-Associated Factor and DNA Replication-related Element binding Factor, named BED hereafter) are among the most frequently found IDs. Five BED-NLRs conferring resistance against bacterial and fungal pathogens have been characterized. However, it is unknown whether BED-NLRs function in a manner similar to other NLR-IDs. Here, we used chromosome-level assemblies of wheat to explore the Yr7 and Yr5a genomic regions and show that, unlike known NLR-ID loci, there is no evidence for a NLR-partner in their vicinity. Using neighbor-network analyses, we observed that BED domains from BED-NLRs share more similarities with BED domains from single-BED proteins and from BED-containing proteins harboring domains that are conserved in transposases. We identified a nuclear localization signal (NLS) in Yr7, Yr5, and the other characterized BED-NLRs. We thus propose that this is a feature of BED-NLRs that confer resistance to plant pathogens. We show that the NLS was functional in truncated versions of the Yr7 protein when expressed in N. benthamiana. We did not observe cell-death upon the overexpression of Yr7 full-length, truncated, and ‘MHD’ variants in N. benthamiana. This suggests that either this system is not suitable to study BED-NLR signaling or that BED-NLRs require additional components to trigger cell death. These results define novel future directions to further understand the role of BED domains in BED-NLR mediated resistance. View Full-Text
Keywords: NLR; integrated domain; NLR-ID; BED-NLR; plant disease resistance; zf-BED; wheat; Yr5; Yr7 NLR; integrated domain; NLR-ID; BED-NLR; plant disease resistance; zf-BED; wheat; Yr5; Yr7
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MDPI and ACS Style

Marchal, C.; 10+ Wheat Genome Project; Haberer, G.; Spannagl, M.; Uauy, C. Comparative Genomics and Functional Studies of Wheat BED-NLR Loci. Genes 2020, 11, 1406. https://doi.org/10.3390/genes11121406

AMA Style

Marchal C, 10+ Wheat Genome Project, Haberer G, Spannagl M, Uauy C. Comparative Genomics and Functional Studies of Wheat BED-NLR Loci. Genes. 2020; 11(12):1406. https://doi.org/10.3390/genes11121406

Chicago/Turabian Style

Marchal, Clemence, 10+ Wheat Genome Project, Georg Haberer, Manuel Spannagl, and Cristobal Uauy. 2020. "Comparative Genomics and Functional Studies of Wheat BED-NLR Loci" Genes 11, no. 12: 1406. https://doi.org/10.3390/genes11121406

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