Next Article in Journal
High Rates of Three Common GJB2 Mutations c.516G>C, c.-23+1G>A, c.235delC in Deaf Patients from Southern Siberia Are Due to the Founder Effect
Next Article in Special Issue
Comparative Genomics and Functional Studies of Wheat BED-NLR Loci
Previous Article in Journal
Computational Analysis of the Global Effects of Ly6E in the Immune Response to Coronavirus Infection Using Gene Networks
Previous Article in Special Issue
NLGenomeSweeper: A Tool for Genome-Wide NBS-LRR Resistance Gene Identification
Article

Deciphering the Impact of a Bacterial Infection on Meiotic Recombination in Arabidopsis with Fluorescence Tagged Lines

by 1,2 and 1,2,*
1
Institute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, CNRS, INRAE, Univ Evry, 91405 Orsay, France
2
Institute of Plant Sciences Paris-Saclay (IPS2), Université de Paris, CNRS, INRAE, 91405 Orsay, France
*
Author to whom correspondence should be addressed.
Genes 2020, 11(7), 832; https://doi.org/10.3390/genes11070832
Received: 14 May 2020 / Revised: 25 June 2020 / Accepted: 10 July 2020 / Published: 21 July 2020
(This article belongs to the Special Issue NLR Gene Evolution in Plants)
Plants are under strong evolutionary pressure to maintain surveillance against pathogens. One major disease resistance mechanism is based on NB-LRR (NLR) proteins that specifically recognize pathogen effectors. The cluster organization of the NLR gene family could favor sequence exchange between NLR genes via recombination, favoring their evolutionary dynamics. Increasing data, based on progeny analysis, suggest the existence of a link between the perception of biotic stress and the production of genetic diversity in the offspring. This could be driven by an increased rate of meiotic recombination in infected plants, but this has never been strictly demonstrated. In order to test if pathogen infection can increase DNA recombination in pollen meiotic cells, we infected Arabidopsis Fluorescent Tagged Lines (FTL) with the virulent bacteria Pseudomonas syringae. We measured the meiotic recombination rate in two regions of chromosome 5, containing or not an NLR gene cluster. In all tested intervals, no significant difference in genetic recombination frequency between infected and control plants was observed. Although it has been reported that pathogen exposure can sometimes increase the frequency of recombinant progeny in plants, our findings suggest that meiotic recombination rate in Arabidopsis may be resilient to at least some pathogen attack. Alternative mechanisms are discussed. View Full-Text
Keywords: biotic stress; meiotic recombination; NLR genes; Arabidopsis; Fluorescent Tagged Lines; Pseudomonas syringae biotic stress; meiotic recombination; NLR genes; Arabidopsis; Fluorescent Tagged Lines; Pseudomonas syringae
Show Figures

Figure 1

MDPI and ACS Style

Gratias, A.; Geffroy, V. Deciphering the Impact of a Bacterial Infection on Meiotic Recombination in Arabidopsis with Fluorescence Tagged Lines. Genes 2020, 11, 832. https://doi.org/10.3390/genes11070832

AMA Style

Gratias A, Geffroy V. Deciphering the Impact of a Bacterial Infection on Meiotic Recombination in Arabidopsis with Fluorescence Tagged Lines. Genes. 2020; 11(7):832. https://doi.org/10.3390/genes11070832

Chicago/Turabian Style

Gratias, Ariane, and Valérie Geffroy. 2020. "Deciphering the Impact of a Bacterial Infection on Meiotic Recombination in Arabidopsis with Fluorescence Tagged Lines" Genes 11, no. 7: 832. https://doi.org/10.3390/genes11070832

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop