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Article

Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery

1
Department of Biomedical Science & Engineering, Konkuk University, Seoul 05029, Korea
2
Department of Bio-Industrial Technologies, Konkuk University, Seoul 05029, Korea
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(11), 1306; https://doi.org/10.3390/genes11111306
Received: 22 October 2020 / Revised: 2 November 2020 / Accepted: 2 November 2020 / Published: 4 November 2020
(This article belongs to the Section Microbial Genetics and Genomics)
Baculovirus expression systems have been widely used to produce recombinant mammalian proteins owing to the lack of viral replication in vertebrates. Although several lines of evidence have demonstrated impacts of baculovirus infection in mammalian hosts, genome-wide effects have not been fully elucidated. Here, we provide comparative transcriptome profiles of baculovirus and host-immune response genes in recombinant baculovirus-infected mammalian and insect cells. Specifically, to decipher the impacts of baculovirus infection in mammalian cells, we conducted total RNA-seq on human 293TT cells and insect Sf9 cells infected with recombinant baculovirus. We found that baculovirus genes were rarely expressed under the control of baculoviral promoters in 293TT cells. Although some baculovirus early genes, such as PE38 and IE-01, showed limited expression in 293TT cells, baculoviral late genes were mostly silent. We also found modest induction of a small number of mammalian immune response genes associated with Toll-like receptors, cytokine signaling, and complement in baculovirus-infected 293TT cells. These comprehensive transcriptome data will contribute to improving recombinant baculovirus as tools for gene delivery, gene therapy, and vaccine development. View Full-Text
Keywords: recombinant baculovirus; total RNA-seq; viral gene expression; immune response recombinant baculovirus; total RNA-seq; viral gene expression; immune response
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MDPI and ACS Style

Shin, H.Y.; Choi, H.; Kim, N.; Park, N.; Kim, H.; Kim, J.; Kim, Y.B. Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery. Genes 2020, 11, 1306. https://doi.org/10.3390/genes11111306

AMA Style

Shin HY, Choi H, Kim N, Park N, Kim H, Kim J, Kim YB. Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery. Genes. 2020; 11(11):1306. https://doi.org/10.3390/genes11111306

Chicago/Turabian Style

Shin, Ha Youn, Hanul Choi, Nahyun Kim, Nayoung Park, Heesun Kim, Jaebum Kim, and Young Bong Kim. 2020. "Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery" Genes 11, no. 11: 1306. https://doi.org/10.3390/genes11111306

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