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Open AccessArticle

The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

1
School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea
2
Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 56212, Korea
3
School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
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Department of Pharmaceutical Engineering, Woosuk University, Wanju 55338, Korea
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Department of Integrative Biology, Kyungpook National University, Daegu 41566, Korea
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Departments of Bacteriology and Genetics, University of Wisconsin, Madison, WI 53706, USA
7
Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea
*
Authors to whom correspondence should be addressed.
Genes 2020, 11(1), 103; https://doi.org/10.3390/genes11010103
Received: 12 November 2019 / Revised: 24 December 2019 / Accepted: 13 January 2020 / Published: 16 January 2020
(This article belongs to the Special Issue Omics Studies Focused on Fungal Secondary Metabolism)
The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans. View Full-Text
Keywords: VosA; sexual development; ascospores; Aspergillus nidulans VosA; sexual development; ascospores; Aspergillus nidulans
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Kim, M.-J.; Lee, M.-K.; Pham, H.Q.; Gu, M.J.; Zhu, B.; Son, S.-H.; Hahn, D.; Shin, J.-H.; Yu, J.-H.; Park, H.-S.; Han, K.-H. The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans. Genes 2020, 11, 103.

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