Next Article in Journal
Synergistic Activity of Mobile Genetic Element Defences in Streptococcus pneumoniae
Previous Article in Journal
BACE1 Inhibition Using 2’-OMePS Steric Blocking Antisense Oligonucleotides
Open AccessArticle

Transcriptional Analysis of FOXO1, C/EBP-α and PPAR-γ2 Genes and Their Association with Obesity-Related Insulin Resistance

1
Biomedical Research Institute of Malaga (IBIMA), Faculty of Science, University of Malaga, 29010 Málaga, Spain
2
Department of Endocrinology and Nutrition, Virgen de la Victoria University Hospital, University of Malaga (IBIMA), 29010 Málaga, Spain
3
CIBEROBN (CIBER in Physiopathology of Obesity and Nutrition CB06/03/0018), “Instituto de Salud Carlos III”, 28029 Madrid, Spain
4
CIBERCV (CIBER in cardiovascular diseases), “Instituto de Salud Carlos III”, 28029 Madrid, Spain
5
Unidad de Gestión Clínica Área del Corazón, Virgen de la Victoria University Hospital, University of Malaga (IBIMA), 29010 Málaga, Spain
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(9), 706; https://doi.org/10.3390/genes10090706
Received: 26 July 2019 / Revised: 28 August 2019 / Accepted: 10 September 2019 / Published: 12 September 2019
Background: Obesity is associated with several comorbid disorders, ranging from cardiovascular diseases to insulin resistance. In this context, visceral adipose tissue (VAT) seems to have a close connection with insulin resistance. In our study, we hypothesized that the expression profile of key adipogenic genes, such as proliferator-activated receptor γ type 2 (PPAR-γ2), CCAAT/enhancer-binding protein type α (C/EBP-α), and forkhead box protein class O type 1 (FOXO1) in VAT should shed light on their association with obesity-related insulin resistance. Methods: To test this idea, we studied the expression profile of C/EBP-α, FOXO1 and PPAR-γ2 in VAT from non-obese individuals, and low insulin (LIR-MO) and high insulin morbidly obese (HIR-MO) subjects, through a combination of RT-qPCR, co-immunoprecipitation, ELISA, Western blot analysis and EMSA assays. Results: Our results show that C/EBP-α and PPAR-γ2 were down-expressed in HIR-MO individuals, while FOXO1 was overexpressed. In addition, the PPAR-γ2–RXR-α heterodimer showed weak activity and bound weakly to the putative IGFBP-2–PPRE promoter sequence in VAT from HIR-MO subjects when compared with LIR-MO individuals. Conclusions: These results show that PPAR-γ2, C/EBP-α, FOXO1 and IGFBP-2 have a close relationship with insulin resistance in VAT of morbidly obese individuals. View Full-Text
Keywords: C/EBP-α; PPAR-γ2; FOXO1; IGFBP-2; obesity; insulin resistance C/EBP-α; PPAR-γ2; FOXO1; IGFBP-2; obesity; insulin resistance
Show Figures

Graphical abstract

MDPI and ACS Style

Boughanem, H.; Cabrera-Mulero, A.; Millán-Gómez, M.; Garrido-Sánchez, L.; Cardona, F.; Tinahones, F.J.; Moreno-Santos, I.; Macías-González, M. Transcriptional Analysis of FOXO1, C/EBP-α and PPAR-γ2 Genes and Their Association with Obesity-Related Insulin Resistance. Genes 2019, 10, 706.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop