Next Article in Journal
Reconstruction and Analysis of Gene Networks of Human Neurotransmitter Systems Reveal Genes with Contentious Manifestation for Anxiety, Depression, and Intellectual Disabilities
Next Article in Special Issue
Molecular Alterations in Thyroid Cancer: From Bench to Clinical Practice
Previous Article in Journal
BRCA2 Deletion Induces Alternative Lengthening of Telomeres in Telomerase Positive Colon Cancer Cells
Previous Article in Special Issue
Thyroid Cancer: The Quest for Genetic Susceptibility Involving DNA Repair Genes

Genes 2019, 10(9), 698; https://doi.org/10.3390/genes10090698

Article
Twenty-Five Years Experience on RET Genetic Screening on Hereditary MTC: An Update on The Prevalence of Germline RET Mutations
Department of Clinical and Experimental Medicine, Unit of Endocrinology University of Pisa, 56124 Pisa, Italy
*
Author to whom correspondence should be addressed.
Received: 8 July 2019 / Accepted: 9 August 2019 / Published: 10 September 2019

Abstract

:
Background: Pathogenic germline mutations affecting the RET proto-oncogene underlie the development of hereditary medullary thyroid carcinoma (MTC). The aims of this study were to evaluate the prevalence of germline RET mutations in a large series of MTC, collected over the last 25 years, and to reappraise their clinical significance. Methods: We performed RET genetic screening in 2031 Italian subjects: patients who presented with sporadic (n = 1264) or hereditary (n = 117) MTC, plus 650 relatives. Results: A RET germline mutation was found in 115/117 (98.3%) hereditary and in 78/1264 (6.2%) apparently sporadic cases: in total, 42 distinct germline variants were found. The V804M mutation was the most prevalent in our cohort, especially in cases that presented as sporadic, while mutations affecting cysteine residues were the most frequent in the group of clinically hereditary cases. All M918T mutations were “de novo” and exclusively associated with MEN2B. Several variants of unknown significance (VUS) were also found. Conclusions: a) RET genetic screening is informative in both hereditary and sporadic MTC; b) the prevalence of different mutations varies with V804M being the most frequent; c) the association genotype–phenotype is confirmed; d) by RET screening, some VUS can be found but their pathogenic role must be demonstrated before screening the family.
Keywords:
medullary thyroid carcinoma; genetic screening; RET; VUS

1. Introduction

Medullary Thyroid Carcinoma (MTC) can be either sporadic or hereditary. The hereditary form gives origin to the Multiple Endocrine Neoplasia (MEN) disorders in which thyroid carcinoma can be associated with additional endocrine neoplasia such as pheochromocytoma and/or hyperparathyroidism (MEN2A) and with non endocrine diseases such as mucosal neuromas, megacolon, marfanoid habitus and skeletal abnormalities (MEN2B). A third form of hereditary MTC is represented by familial MTC (FMTC), which is characterized by MTC alone, but in several relatives of the same family [1,2]. The RET proto-oncogene encodes for a tyrosine kinase transmembrane receptor that regulates cell growth, proliferation, survival and differentiation [3]. The oncogenic transforming role of germline RET mutations in hereditary medullary thyroid carcinoma (MTC) has been reported 25 years ago [4]. Following the first description of point mutations in MTC the prevalence of these alterations and the correlation with different phenotypes have been extensively described and discussed in outstanding reviews [5,6,7]. In addition to the classical cysteine mutations, which were originally shown to be associated with the multiple endocrine neoplasia type 2A (MEN2A) [4] and the M918T mutation that is causative of MEN2B [8], over the years additional RET mutations have been discovered mainly in FMTC. These mutations occur mostly in cysteines, but can also affect non-cysteine codons, mainly at codons E768 [9], L790 and S791 in exon 13 [10], codon V804 in exon 14 [11] and codons A883 [12] and S891 in exon 15 [13]. Very rare RET mutations, of uncertain impact in disease pathogenesis (“variants of unknown significance”, VUS), have also been reported often in a single subject or family [14,15,16].
RET germline mutations have been found in almost all MEN2 families, with only about 2% of families affected by hereditary MTC having no detectable germline mutations [7,17]. RET germline mutations, mainly affecting non-cysteine codons, have been reported in about 5–10% of apparently sporadic MTC cases [18,19,20]. At the tumor level, somatic RET mutations, commonly point mutations but also deletions/insertions, have been described in about 50% of the sporadic MTC, according to data published in the COSMIC database [21]. In sporadic MTC, the presence of a somatic RET mutation, particularly M918T, has been found to correlate with more aggressive tumor features, a more advanced stage at diagnosis and a worse prognosis [22,23].
Although codon 634 mutations are the most prevalent mutations in both European and non-European families [24,25], mutations at codon 533 are dominant in Greece [26] and mutations at codon 618 are particularly frequent in Cyprus [27]. A recent large Italian study showed a high prevalence of codon 804 mutations [28] whereas a high prevalence of codon 790 mutations has been noted in Germany [10].
The introduction of RET genetic screening in clinical practice for hereditary MTC favorably modified the clinical management of MTC patients. According to the recent American [29] and European guidelines [30], all patients with MTC should undergo RET genetic screening to identify both hereditary cases erroneously diagnosed as sporadic and RET mutation carriers in MEN2 families. Relatives who are RET negative will not develop MTC, whereas RET-positive subjects are at risk to develop the cancer and must be followed and treated [29,31].
The aim of this study was to provide an update on the prevalence of germline RET mutations in our large series of sporadic and hereditary MTC after 25 years of genetic screening. A reappraisal of the clinical meaning of the RET mutations is also included.

2. Patients and Methods

2.1. Subjects

During the last 25 years (1993-2018), we have performed RET genetic screening in a total of 2031 Italian subjects: 117 were clinically affected by a hereditary disease at diagnosis, 1264 were affected by a sporadic MTC form (i.e., no familial MTC history and no other endocrine diseases), 650 subjects were relatives of RET positive MTC patients (Figure 1). Informed consent for RET genetic screening and other clinical procedures was obtained from all investigated subjects. The present study has been approved by the local ethical committee.

2.2. RET Genetic Analysis

Blood has been collected over this 25-years period and stored at −20 °C. Over the years, the procedure of DNA extraction and sequencing analysis has been modified; until 2006, genomic DNA was purified from peripheral blood lymphocytes using the QIAMP blood kit (QIAGEN, Hilden, Germany). From 2006 until today an automated method (Maxwell16 Instrument, Promega, Madison, WI, USA) for genomic DNA extraction has been introduced. During this time interval also the RET sequencing has been modified: in the first years only exons 10, 11 and 16 were analyzed. Following the identification of additional RET mutation spread in a larger portion of the gene the analysis of exons 5, 8, 13, 14 and 15 has been introduced. With the exception of relatives of RET positive index cases, who have been analyzed only for the presence of the mutation identified in their family, all patients included in the study have been screened for the presence of RET mutations in eight exons (5, 8, 10, 11, 13, 14, 15 and 16). In familial cases with no evidence of RET mutations in these commonly analyzed exons, the entire coding sequence of the RET gene was analyzed. The biological rationale to focus on this region of the RET gene is that these mutations cause ligand-independent constitutive activation of the tyrosine kinase receptor. Cysteine mutations induce the formation of disulphide-bonded homodimers while mutations in the tyrosine kinase domain of the receptor, in particular the M918T mutation, cause a constitutive high level of autophosphorylation. Both mechanisms lead to activation of downstream signaling pathways, independent of ligand binding to the receptor.
Currently, genomic DNA is amplified using KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, Saint Louis, MO, USA) in a final volume of 25 μL (My Cycle instrument, Biorad, Hercules, CA, USA). Amplification cycle is performed with an initial step of 95 °C for 2 min, followed by 35 cycles of 95 °C for 15 s, 60 °C for 15 s and 72 °C for 15 s. A final extension at 72 °C for 7 min was performed at the end of the amplification protocol. Sequence analysis was performed and has been reported on previously [32]. Primers’sequences can be provided upon request.

3. Results

Among the 2031 subjects submitted to RET genetic screening in the period 1993-2018 (25 yrs), 117 were clinically affected by a hereditary disease at diagnosis: 115/117 (98.3%) were found to be carriers of a RET germline mutation, while two families (1.7%) had no detectable RET mutations. A germline RET mutation was also found in 78/1264 (6.2%) MTC patients who were diagnosed as sporadic according to their negative familial history and absence of other endocrine neoplasms. Considering the cases found to be familial at diagnosis (clinically familial cases) and familial cases erroneously diagnosed as sporadic (apparently sporadic cases) a total of 195 families with hereditary MTC were followed at our hospital. The remaining 1186 sporadic MTC patients were found to be negative for germline RET mutations and their sporadic nature has been confirmed.
Following the identification of a RET mutation in the index cases, 650 relatives have been analyzed and 256 (40%) subjects were found to carry the same RET mutation as their affected family members. The remaining 394 (60%) individuals were RET negative (Figure 1). In the whole series, 449 subjects have been found to harbour a RET germline mutations. RET mutations that are inherited as autosomal dominant Mendelian traits, were present in about 50% of family members. An exception to this prevision is represented by the MEN2B-associated M918T mutation, which was present in about 30% of our patients and was found to be a de novo germline mutation in all cases. In addition to the MEN2B patients, seven MEN2A-associated mutations were proven to be “de novo”. Five of these cases had a C634X, 1 had a C618S and the last a C620S germline mutation.
All together 42 different germline missense mutations have been identified (Figure 2). The V804M RET mutation was the most prevalent missense variant in our series, both when considering all cases together (50/195, 25.6%) and when considering clinically familial (23/117, 19.6%) or apparently sporadic (27/78, 34.6%) cases. When taken together, mutations affecting the cysteine at codon 634 (C634R/Y/G/F/W/S) were found in 45/195 cases (23%). Cys634 mutations, were the most prevalent germline mutations in the group of clinically familial cases (37/117, 31.6%) and were significantly (p = 0.0005) more frequent in this group than in the group of apparently sporadic cases (8/78, 10.2%). When considering all cysteine mutations together a significantly higher prevalence was observed in clinically familial cases than in apparently sporadic cases (54/117 (46.1%) vs 23/78 (29.5%), respectively) (p = 0.02). Conversely, germline mutations affecting non-cysteine residues were more frequent in apparently sporadic than in clinically familial cases (55/78 (69.2%) vs 61/117, (52%), respectively) (p = 0.01). The M918T mutation, found in 15/117 (12.8%) cases, was always a de novo mutation and the patients were all affected by MEN2B syndrome.
Among all mutations, we found some rare variants (R215L, K666Q, K821E, V871I, T338I, E632K, V648I, R833C, M848T, A883T) previously reported but of unknown biological significance (VUS) (Table 1). The VUS were found in 3/117 (2.5%) cases of familial MTC and in 11/78 (14.1%) apparently sporadic cases. In 4 families these variants were found only in the index cases and in eight cases more than one family member was carrying the RET mutation but only the index case presented with MTC so far (Figure 3). Moreover, very rare germline synonymous mutations, which did not modify the encoded aminoacid (GAC->GAT, D631D; AAC->AAT, N763N; TTC->TTT, F893F) were found in three clinically sporadic cases and, so far, their role in MTC pathogenesis, if any, is undefined.
As shown in Table 2, among the 45 families with a C634 mutation in exon 11, 32 (71.1%) had PHEO (pheochromocytoma) and in six cases (13.3%) also hyperPTH (hyperparathyroidism). Among the families with a cysteine mutation in exon 10, PHEO or hyperPTH were present in 5/28 (17.8%) families. In the M918T cases affected with MEN2B, PHEO was present in 4/15 and Marphan syndrome and/or mucosal neurinomas were present in all cases while none of them had hyperPTH. Among families with non-cysteine mutation only two, both carrying a V804M mutation showed the association of MTC and PHEO in several family members. In a third V804M family, the association of MTC with hyperPTH was observed in one single individual. Three MEN2A families showed as association with the Hirshprungs’ disease and carried a RET germline mutation in exon 10 (C609R, C618R and C620Y).

4. Discussion

Soon after the discovery of the driving role of RET mutations in the pathogenesis of MTC [4,8] RET genetic screening in patients with hereditary and sporadic MTC has been introduced in the clinical practice to identify RET positive subjects and their relatives who are at high risk of developing MTC during their life.
Over the last 25 years, we have screened the RET gene in a series of 2031 Italian subjects including 1381 MTC patients and 650 relatives. As a standard routine procedure, only RET exons reported to be causative of the inherited MTC have been investigated (exons 5, 8, 10, 11, 13, 14, 15 and 16). Furthermore, in patients with familial MTC who were negative on the first screen, the whole RET coding sequence has been analyzed. In agreement with previously reported data we have found that about 98.5% of hereditary MTC are affected by a RET germline mutation but a few cases are still RET negative despite the screening covered all RET exons. To explain the hereditary nature of RET cases, the presence of a mutation in genes other than RET could be hypothesized or the presence of a RET intronic variant with the ability to modify RET expression levels could be considered [33].
In addition, and in keeping with other reports [18,34], we demonstrated the presence of a germline RET mutation in more than 6% of apparently sporadic MTC patients, thus confirming the important role of RET genetic screening for the identification of unsuspected MEN2 families [18]. Once an apparently sporadic MTC patient is reclassified as hereditary, RET genetic screening should be performed in all first-degree family members, allowing the identification of gene carriers who can thus benefit from appropriate screening, an early diagnosis and timely or prophylactic treatment measures.
In 1994 a study performed by the International RET Consortium (IRC) [35] analyzed 477 MEN 2 kindred and demonstrated that about 94% of cases presented with a RET germline mutation affecting one of the following codons: 609, 611, 618, 620, 634, 768, 804 and 918. In the present series, the percentage of RET positive families (193/195, 98.9%) was higher than that reported in the IRC study. This finding could be explained by the inclusion of a wider portion of the RET gene in the screen (to include additional variants identified since the publication of the IRC study), and perhaps to improvements in sequencing technologies. In fact, over the past 25 years, 197 different RET variants, (some pathogenic and others VUS), have been associated with MEN2 [16] and most of the cases that were originally considered negative for RET germline variants have been reanalyzed and reclassified as positive. Our wide RET screening approach identified 42 different point mutations. In our series, the most frequent RET mutation was the V804M in exon 14. Our findings support previously reported data [28,36] indicating that V804M is the most frequent RET variant associated with hereditary MTC in Italy, while mutations at codon 634 have been found to be the most frequent in Germany [37] and in other European and non-European countries [24,25]. The high prevalence of the V804M mutation in Italy is likely the result of a “founder” effect that amplified the prevalence of this specific, relatively uncommon mutation in the population.
The present study describes a different profile of RET germline mutations between the group of the clinically hereditary cases and the cohort of apparently sporadic cases. Although the V804M mutation is the most frequent single germline mutation observed in the two groups, mutations involving the cysteine at codon 634, and overall, mutations involving the cysteine residues in exons 10 and 11, are significantly more frequent in the group of clinically hereditary cases than in apparently sporadic cases. Conversely, mutations affecting non-cysteine residues are more frequent in apparently sporadic cases than in the clinically familial cases. This difference can be explained by the higher oncogenic potential and penetrance of the cysteine mutations, with particular regard to C634R, when compared to non-cysteine RET variants [15]. The different biological behavior of the mutations is responsible for a difference in the latency period and aggressiveness of the MTC and in the prevalence of the associated endocrine diseases (PHEO and hyperPTH) making the syndromes determined by cysteine mutations easier to be clinically identified [7,38].
Among all mutations, we found some rare variants whose biological significance is still unknown (VUS). The accurate characterization of the pathogenic role of these variants would be of great relevance for patients. The aggressiveness and the biological role of these new variants can be predicted by “in silico” on line tools [14,15] even if sometimes, the predicted results do not correlate with the in vitro assay. For this reason, clinicians should be cautious in trusting computational results to design appropriate treatment for cancer patients with these VUS. Among the 42 different RET germline mutations of our series, the Y791F mutation is still reported although recent studies indicate that this mutation does not increase the risk for MTC [39]. In agreement with these reported evidences, MTC was completely absent in one family with 3 positive members while in the other two only the index cases showed the MTC. Our interpretation is that in these latter two families, the index case had the MTC for other genetic reasons, likely for sporadic alterations, and the identification of the germline mutation was a “non-target” effect of the RET screening.
In our series the MEN2B patients were all affected by the M918T mutations in exon 16 of the RET gene that it is known to account for 95% of MEN2B cases [8,40]. As reported in other series [41], also in our cases the RET mutations in MEN2B were all de novo. In addition to the MEN2B cases, seven additional RET positive patients have been documented to be de novo and in these cases RET mutations were discovered in exon 10 or 11. The finding of de novo mutations in MEN2A is of interest and scarcely reported and discussed. Proving the de novo origin of these mutations would require the analysis of both parents and this is not always possible, especially in adult index cases whose parents may have already died. Moreover, since is not ethically acceptable to test for paternity, doubts about the progenitorship cannot be ruled out. However, it is conceivable that, as it happens for MEN2B [42], also in a subgroup of MEN2A and likely also of FMTC, the RET mutation happened as “somatic” event in one of the gametes or in the early phases of embryonic development.
In agreement with previous series [20,43], a strong genotype–phenotype correlation has been confirmed in our Italian cohort. In particular, the observation that MTC is strongly associated to RET mutations strengthens the idea that alterations in the RET gene are enough to induce tumoral transformation as indeed demonstrated by in vitro studies [44]. PHEO and/or hyperPTH are mainly associated with cysteine mutations, in particular at Cys634, and are very rare in families with non-cysteine RET mutations. In our series only three families with a non-cysteine RET mutation had members with PHEO or hyperPTH and were all harboring a V804M mutation. This evidence is relevant for clinicians because the follow up of patients with non-cysteine RET mutations could be simplified by eliminating the annual evaluations of adrenal gland and parathyroid function which are still recommended in the clinical practice guidelines. Of course, it remains mandatory in cases with cysteine mutations, particularly when a C634R is present.
Hirschsprung’s disease is also associated with RET mutations but, in contrast to those associated with MTC, they are inactivating mutations [45,46]. In some cases, mutations in RET C620, C618, C611 and C609, act as “Janus” mutations [47]: that are able to induce both Hirschsprung’s disease and MTC in the same patient or in the same family. These two diseases are present also in some members of three families of our series harbouring typical (cysteine-associated) RET “Janus” mutations. However, we have identified other two families with non-cysteine RET mutations, (K821E and Y791F) with one single member with Hirschsprung’s disease and, so far, no one with MTC. It is interesting that in both cases the mutations are in the intracellular domain of the receptor, in particular in exon 13 and 14, that are commonly screened for MTC but never demonstrated to be Janus mutation. Only the long-term follow up of these young patients and their RET positive relatives will clarify if they will ever develop MTC.

5. Conclusions

In this study, that to our knowledge is the largest series of screened MTC at a single center, we showed that: (a) RET genetic screening should be performed in all MTC cases independently from their clinical presentation; (b) V804M is confirmed to be the most frequent RET mutation in the Italian population; (c) cysteine mutations are more frequent in cases with a clinically manifested hereditary form; (d) the non-cysteine mutations are very rarely associated with PHEO and hyperPTH suggesting that the follow up of these patients could be simplified; (e) de novo mutations are frequent in MEN2B syndrome but they can be found also in both MEN2A and FMTC; (f) germline RET VUS may be found with genetic screening but their pathogenic role should be defined before testing relatives of the index case.

Author Contributions

R.E. and C.R. participated in the study for all the 25 years, they took care of the recruitment of patients, of the analysis of the results and of the preparation of the manuscript. A.T., R.C., T.R. were in charge of the bench work; all the other authors were clinicians who have followed our patients in this period.

Acknowledgments

This study has been supported by Associazione Italiana Ricerca sul Cancro (IG 2018, cod 21790); Agenzia Italiana del Farmaco (Cod AIFA-2016-02365049); PRA_2018_27“ Studio del profilo di progressione tumorale nei carcinomi midollari tiroidei e paratiroidei”.

Conflicts of Interest

The authors declare that there is no conflict of interest that could affect the impartiality of the reported research.

References

  1. Romei, C.; Pardi, E.; Cetani, F.; Elisei, R. Genetic and clinical features of multiple endocrine neoplasia types 1 and 2. J. Oncol. 2012, 2012, 15. [Google Scholar] [CrossRef]
  2. Wohllk, N.; Schweizer, H.; Erlic, Z.; Schmid, K.W.; Walz, M.K.; Raue, F.; Neumann, H.P.H. Multiple endocrine neoplasia type 2. Best Pract. Res. Clin. Endocrinol. Metab. 2010, 24, 371–387. [Google Scholar] [CrossRef] [PubMed]
  3. Mulligan, L.M. GDNF and the RET Receptor in Cancer: New Insights and Therapeutic Potential. Front. Physiol. 2018, 9, 1873. [Google Scholar] [CrossRef] [PubMed]
  4. Mulligan, L.M.; Kwok, J.B.J.; Healey, C.S.; Elsdon, M.J.; Eng, C.; Gardner, E.; Love, D.R.; Mole, S.E.; Moore, J.K.; Papi, L.; et al. Germ-line mutations of the RET proto-oncogene in multiple endocrine neoplasia type 2A. Nature 1993, 363, 458–460. [Google Scholar] [CrossRef] [PubMed]
  5. Mulligan, L.M. RET revisited: Expanding the oncogenic portfolio. Nat. Rev. Cancer 2014, 14, 173–186. [Google Scholar] [CrossRef] [PubMed]
  6. Wells, S.A.J. Advances in the management of MEN2: From improved surgical and medical treatment to novel kinase inhibitors. Endocr. Relat. Cancer 2018, 25, T1–T13. [Google Scholar] [CrossRef]
  7. Romei, C.; Ciampi, R.; Elisei, R. A comprehensive overview of the role of the RET proto-oncogene in thyroid carcinoma. Nat. Rev. Endocrinol. 2016, 12, 192–202. [Google Scholar] [CrossRef]
  8. Hofstra, R.M.W.; Landsvater, R.M.; Ceccherini, I.; Stulp, R.P.; Stelwagen, T.; Luo, Y.; Pasini, B.; Höppener, J.W.M.; Van Amstel, H.K.P.; Romeo, G.; et al. A mutation in the RET proto-oncogene associated with multiple endocrine neoplasia type 2B and sporadic medullary thyroid carcinoma. Nature 1994, 367, 375–376. [Google Scholar] [CrossRef]
  9. Antinolo, G.; Marcos, I.; Fernandez, R.M.; Romero, M.; Borrego, S. A novel germline point mutation, c.2304 G-->T, in codon 768 of the RET proto-oncogene in a patient with medullary thyroid carcinoma. Am. J. Med. Genet. 2002, 110, 85–87. [Google Scholar] [CrossRef]
  10. Berndt, I.; Reuter, M.; Saller, B.; Frank-Raue, K.; Groth, P.; Grussendorf, M.; Raue, F.; Ritter, M.M.; Hoppner, W. A New Hot Spot for Mutations in the ret Protooncogene Causing Familial Medullary Thyroid Carcinoma and Multiple Endocrine Neoplasia Type 2A. J. Clin. Endocrinol. Metab. 1998, 83, 770–774. [Google Scholar] [CrossRef]
  11. Fink, M.; Weinhäusel, A.; Niederle, B.; Haas, O.A. Distinction between sporadic and hereditary medullary thyroid carcinoma (MTC) by mutation analysis of the RET proto-oncogene. Int. J. Cancer 1996, 69, 312–316. [Google Scholar] [CrossRef]
  12. Marsh, D.J.; Learoyd, D.L.; Andrew, S.D.; Krishnan, L.; Pojer, R.; Richardson, A.L.; Delbridge, L.; Eng, C.; Robinson, B.G. Somatic mutations in the RET proto-oncogene in sporadic medullary thyroid carcinoma. Clin. Endocrinol. 1996, 44, 249–257. [Google Scholar] [CrossRef] [PubMed]
  13. Dang, G.T.; Cote, G.J.; Schultz, P.N.; Khorana, S.; Decker, R.A.; Gagel, R.F. A codon 891 exon 15 RET proto-oncogene mutation in familial medullary thyroid carcinoma: A detection strategy. Mol. Cell. Probes 1999, 13, 77–79. [Google Scholar] [CrossRef] [PubMed]
  14. Crockett, D.K.; Piccolo, S.R.; Ridge, P.G.; Margraf, R.L.; Lyon, E.; Williams, M.S.; Mitchell, J.A. Predicting Phenotypic Severity of Uncertain Gene Variants in the RET Proto-Oncogene. PLoS ONE 2011, 6, e18380. [Google Scholar] [CrossRef] [PubMed]
  15. Cosci, B.; Vivaldi, A.; Romei, C.; Gemignani, F.; Landi, S.; Ciampi, R.; Tacito, A.; Molinaro, E.; Agate, L.; Bottici, V.; et al. In silico and in vitro analysis of rare germline allelic variants of RET oncogene associated with medullary thyroid cancer. Endocr. Relat. Cancer 2011, 18, 603–612. [Google Scholar] [CrossRef] [PubMed]
  16. Margraf, R.L.; Crockett, D.K.; Krautscheid, P.M.F.; Seamons, R.; Calderon, F.R.O.; Wittwer, C.T.; Mao, R. Multiple endocrine neoplasia type 2 RET protooncogene database: Repository of MEN2-associated RET sequence variation and reference for genotype/phenotype correlations. Hum. Mutat. 2009, 30, 548–556. [Google Scholar] [CrossRef] [PubMed]
  17. Raue, F.; Frank-Raue, K. Update on Multiple Endocrine Neoplasia Type 2: Focus on Medullary Thyroid Carcinoma. J. Endocr. Soc. 2018, 2, 933–943. [Google Scholar] [CrossRef] [PubMed]
  18. Romei, C.; Cosci, B.; Renzini, G.; Bottici, V.; Agate, L.; Passannanti, P.; Viola, D.; Biagini, A.; Materazzi, G.; Pinchera, A.; et al. RET genetic screening of sporadic medullary thyroid cancer (MTC) allows the preclinical diagnosis of unsuspected gene carriers and the identification of a relevant percentage of hidden familial MTC (FMTC). Clin. Endocrinol. 2011, 74, 241–247. [Google Scholar] [CrossRef] [PubMed]
  19. Wiench, M.; Wygoda, Z.; Gubala, E.; Wloch, J.; Lisowska, K.; Krassowski, J.; Scieglinska, D.; Fiszer-Kierzkowska, A.; Lange, D.; Kula, D.; et al. Estimation of Risk of Inherited Medullary Thyroid Carcinoma in Apparent Sporadic Patients. J. Clin. Oncol. 2001, 19, 1374–1380. [Google Scholar] [CrossRef]
  20. Niccoli-Sire, P.; Murat, A.; Rohmer, V.; Franc, S.; Chabrier, G.; Baldet, L.; Maes, B.; Savagner, F.; Giraud, S.; Bezieau, S.; et al. Familial Medullary Thyroid Carcinoma with Noncysteine RET Mutations: Phenotype-Genotype Relationship in a Large Series of Patients. J. Clin. Endocrinol. Metab. 2001, 86, 3746–3753. [Google Scholar] [CrossRef]
  21. Catalogue of Somatic Mutations in Cancer. Available online: https://cancer.sanger.ac.uk (accessed on 13 June 2019).
  22. Romei, C.; Ugolini, C.; Cosci, B.; Torregrossa, L.; Vivaldi, A.; Ciampi, R.; Tacito, A.; Basolo, F.; Materazzi, G.; Miccoli, P.; et al. Low Prevalence of the Somatic M918T RET Mutation in Micro-Medullary Thyroid Cancer. Thyroid 2012, 22, 476–481. [Google Scholar] [CrossRef] [PubMed]
  23. Romei, C.; Casella, F.; Tacito, A.; Bottici, V.; Valerio, L.; Viola, D.; Cappagli, V.; Matrone, A.; Ciampi, R.; Piaggi, P.; et al. New insights in the molecular signature of advanced medullary thyroid cancer: Evidence of a bad outcome of cases with double RET mutations. J. Med. Genet. 2016, 53, 729–734. [Google Scholar] [CrossRef] [PubMed]
  24. Maciel, R.M.B.; Camacho, C.P.; Assumpção, L.V.M.; Bufalo, N.E.; Carvalho, A.L.; De Carvalho, G.A.; Castroneves, L.A.; De Castro, F.M.; Ceolin, L.; Cerutti, J.M.; et al. Genotype and phenotype landscape of MEN2 in 554 medullary thyroid cancer patients: The BrasMEN study. Endocr. Connect. 2019, 8, 289–298. [Google Scholar] [CrossRef] [PubMed]
  25. Machens, A.; Hoegel, J.; Van Vroonhoven, T.J.; Roeher, H.-D.; Wahl, R.A.; Raue, F.; Niccoli-Sire, P.; Frank-Raue, K.; Lamesch, P.; Conte-Devolx, B.; et al. Early Malignant Progression of Hereditary Medullary Thyroid Cancer. N. Engl. J. Med. 2003, 349, 1517–1525. [Google Scholar] [CrossRef] [PubMed]
  26. Sarika, H.L.; Papathoma, A.; Garofalaki, M.; Vasileiou, V.; Vlassopoulou, B.; Anastasiou, E.; Alevizaki, M. High prevalence of exon 8 G533C mutation in apparently sporadic medullary thyroid carcinoma in Greece. Clin. Endocrinol. 2012, 77, 857–862. [Google Scholar] [CrossRef]
  27. Fanis, P.; Skordis, N.; Frangos, S.; Christopoulos, G.; Spanou-Aristidou, E.; Andreou, E.; Manoli, P.; Mavrommatis, M.; Nicolaou, S.; Kleanthous, M.; et al. Multiple endocrine neoplasia 2 in Cyprus: Evidence for a founder effect. J. Endocrinol. Investig. 2018, 41, 1149–1157. [Google Scholar] [CrossRef]
  28. Romei, C.; Mariotti, S.; Fugazzola, L.; Taccaliti, A.; Pacini, F.; Opocher, G.; Mian, C.; Castellano, M.; Degli Uberti, E.; Ceccherini, I.; et al. Multiple endocrine neoplasia type 2 syndromes (MEN 2): Results from the ItaMEN network analysis on the prevalence of different genotypes and phenotypes. Eur. J. Endocrinol. 2010, 163, 301–308. [Google Scholar] [CrossRef]
  29. Wells, S.A.J.; Asa, S.L.; Dralle, H.; Elisei, R.; Evans, D.B.; Gagel, R.F.; Lee, N.; Machens, A.; Moley, J.F.; Pacini, F.; et al. Revised American Thyroid Association guidelines for the management of medullary thyroid carcinoma. Thyroid 2015, 25, 567–610. [Google Scholar] [CrossRef]
  30. Elisei, R.; Alevizaki, M.; Conte-Devolx, B.; Frank-Raue, K.; Leite, V.; Williams, G. 2012 European Thyroid Association Guidelines for Genetic Testing and Its Clinical Consequences in Medullary Thyroid Cancer. Eur. Thyroid J. 2012, 1, 216–231. [Google Scholar] [CrossRef]
  31. Elisei, R.; Romei, C.; Renzini, G.; Bottici, V.; Cosci, B.; Molinaro, E.; Agate, L.; Cappagli, V.; Miccoli, P.; Berti, P.; et al. The Timing of Total Thyroidectomy in RET Gene Mutation Carriers Could Be Personalized and Safely Planned on the Basis of Serum Calcitonin: 18 Years Experience at One Single Center. J. Clin. Endocrinol. Metab. 2012, 97, 426–435. [Google Scholar] [CrossRef]
  32. Elisei, R.; Cosci, B.; Romei, C.; Bottici, V.; Sculli, M.; Lari, R.; Barale, R.; Pacini, F.; Pinchera, A. RET exon 11 (G691S) polymorphism is significantly more frequent in sporadic medullary thyroid carcinoma than in the general population. J. Clin. Endocrinol. Metab. 2004, 89, 3579–3584. [Google Scholar] [CrossRef] [PubMed]
  33. Pecce, V.; Sponziello, M.; Damante, G.; Rosignolo, F.; Durante, C.; Lamartina, L.; Grani, G.; Russo, D.; di Gioia, C.R.; Filetti, S.; et al. A synonymous RET substitution enhances the oncogenic effect of an in-cis missense mutation by increasing constitutive splicing efficiency. PLoS Genet. 2018, 14, e1007678. [Google Scholar] [CrossRef] [PubMed]
  34. Eng, C.; Mulligan, L.M.; Smith, D.P.; Healey, C.S.; Frilling, A.; Raue, F.; Neumann, H.P.; Ponder, M.A.; Ponder, B.A. Low frequency of germline mutations in the RET proto-oncogene in patients with apparently sporadic medullary thyroid carcinoma. Clin. Endocrinol. 1995, 43, 123–127. [Google Scholar] [CrossRef] [PubMed]
  35. Eng, C. The relationship between specific RET proto-oncogene mutations and disease phenotype in multiple endocrine neoplasia type 2. International RET mutation consortium analysis. JAMA 1996, 276, 1575–1579. [Google Scholar] [CrossRef] [PubMed]
  36. Pinna, G.; Orgiana, G.; Riola, A.; Ghiani, M.; Lai, M.L.; Carcassi, C.; Mariotti, S. RET Proto-Oncogene in Sardinia: V804M Is the Most Frequent Mutation and May Be Associated with FMTC/MEN-2A Phenotype. Thyroid 2007, 17, 101–104. [Google Scholar] [CrossRef]
  37. Machens, A.; Dralle, H. Familial prevalence and age of RET germline mutations: Implications for screening. Clin. Endocrinol. 2008, 69, 81–87. [Google Scholar] [CrossRef] [PubMed]
  38. Frank-Raue, K.; Raue, F. Hereditary Medullary Thyroid Cancer Genotype–Phenotype Correlation. Methods Mol. Biol. 2015, 204, 139–156. [Google Scholar]
  39. Toledo, R.A.; Maciel, R.M.B.; Erlic, Z.; Lourenco, D.M.J.; Cerutti, J.M.; Eng, C.; Neumann, H.P.; Toledo, S.P. RET Y791F Variant Does Not Increase the Risk for Medullary Thyroid Carcinoma. Thyroid 2015, 25, 973–974. [Google Scholar] [CrossRef]
  40. Nagal, M.A.; Healey, C.S.; Ponder, M.A.; Gardner, E.; Scheumann, G.F.; Jackson, C.E.; Tunnacllffe, A.; Eng, C.; Smith, D.P.; Mulligan, L.M. Point mutation within the tyrosine kinase domain of the RET proto-oncogene in multiple endocrine neoplasia type 2B and related sporadic tumours. Hum. Mol. Genet. 1994, 3, 237–241. [Google Scholar]
  41. Brauckhoff, M.; Machens, A.; Lorenz, K.; Bjoro, T.; Varhaug, J.E.; Dralle, H. Surgical curability of medullary thyroid cancer in multiple endocrine neoplasia 2B: A changing perspective. Ann. Surg. 2014, 259, 800–806. [Google Scholar] [CrossRef]
  42. Carlson, K.M.; Bracamontes, J.; Jackson, C.E.; Clark, R.; Lacroix, A.; Wells, S.A.; Goodfellow, P.J. Parent-of-origin effects in multiple endocrine neoplasia type 2B. Am. J. Hum. Genet. 1994, 55, 1076–1082. [Google Scholar] [PubMed]
  43. Raue, F.; Frank-Raue, K. Genotype-phenotype correlation in multiple endocrine neoplasia type 2. Clinics (Sao Paulo) 2012, 67, 69–75. [Google Scholar] [CrossRef]
  44. Santoro, M.; Carlomagno, F.; Romano, A.; Bottaro, D.; Dathan, N.; Grieco, M.; Fusco, A.; Vecchio, G.; Matoskova, B.; Kraus, M.; et al. Activation of RET as a dominant transforming gene by germline mutations of MEN2A and MEN2B. Science 1995, 267, 381–383. [Google Scholar] [CrossRef] [PubMed]
  45. Martucciello, G.; Ceccherini, I.; Lerone, M.; Jasonni, V. Pathogenesis of Hirschsprung’s disease. J. Pediatr. Surg. 2000, 35, 1017–1025. [Google Scholar] [CrossRef] [PubMed]
  46. Amiel, J.; Sproat-Emison, E.; Garcia-Barcelo, M.; Lantieri, F.; Burzynski, G.; Borrego, S.; Pelet, A.; Arnold, S.; Miao, X.; Griseri, P.; et al. Hirschsprung disease, associated syndromes and genetics: A review. J. Med. Genet. 2008, 45, 1–14. [Google Scholar] [CrossRef] [PubMed]
  47. Arighi, E.; Popsueva, A.; Degl’Innocenti, D.; Borrello, M.G.; Carniti, C.; Perälä, N.M.; Pierotti, M.A.; Sariola, H. Biological Effects of the Dual Phenotypic Janus Mutation of ret Cosegregating with Both Multiple Endocrine Neoplasia Type 2 and Hirschsprung’s Disease. Mol. Endocrinol. 2004, 18, 1004–1017. [Google Scholar] [CrossRef] [PubMed]
Figure 1. Subjects who underwent RET genetic screening over the last 25 years at our institution: the prevalence of RET mutations according to the first clinical presentations is reported.
Figure 1. Subjects who underwent RET genetic screening over the last 25 years at our institution: the prevalence of RET mutations according to the first clinical presentations is reported.
Genes 10 00698 g001
Figure 2. Prevalence (A) and distribution along the gene (B) of RET germline mutations in our series. In this series 11 mutations were variants of unknown significance (VUS).
Figure 2. Prevalence (A) and distribution along the gene (B) of RET germline mutations in our series. In this series 11 mutations were variants of unknown significance (VUS).
Genes 10 00698 g002
Figure 3. Pedigrees of MEN2 families with germline RET mutation classified as VUS. With the exception of a A883T RET mutated family in which medullary thyroid carcinoma (MTC) was present in two homozygous patients, MTC was present only in one member of the family. PTC: Papillary Thyroid Cancer.
Figure 3. Pedigrees of MEN2 families with germline RET mutation classified as VUS. With the exception of a A883T RET mutated family in which medullary thyroid carcinoma (MTC) was present in two homozygous patients, MTC was present only in one member of the family. PTC: Papillary Thyroid Cancer.
Genes 10 00698 g003
Table 1. Variant of unknown significance: Prevalence in our series and in silico prediction of their transforming ability.
Table 1. Variant of unknown significance: Prevalence in our series and in silico prediction of their transforming ability.
RET MutationFamiliesPATIENTS (n.)In Silico Prediction
StudiedRET PositiveRET NegativeAffectedGene CarriersSIFTPOLYPHENCADDREVELMETA LRMUTATION ASSESSOR
R215L a111010toleratedlikely benignlikely benignbenigntolerated0.medium
K666Q111010deleteriouspossibly damaginglikely benignLikely disease causingdamaginglow
K821E133012deleteriousprobably damaginglikely benignLikely disease causingdamaginglow
V871I143112deleteriousprobably damaginglikely benignLikely disease causingdamagingneutral
T338I132111toleratedbenignlikely benignlikely benigntoleratedmedium
E632K111010toleratedpossibly damaginglikely benignLikely disease causingdamagingmedium
V648I262411toleratedbenignlikely benignLikely disease causingdamagingneutral
R833C122011deleteriousprobably damaginglikely benignLikely disease causingdamagingmedium
M848T122011toleratedprobably damaginglikely benignLikely disease causingdamagingneutral
A883T2149537deleteriousprobably damaginglikely deleteriousLikely disease causingtoleratedneutral
a this patient had a simultaneous C634Y somatic mutation.
Table 2. Prevalence of endocrine and non endocrine clinical phenotypes according to the type of mutation: Only mutations associated with multiple diseases are reported.
Table 2. Prevalence of endocrine and non endocrine clinical phenotypes according to the type of mutation: Only mutations associated with multiple diseases are reported.
RET MutationNumber of FamiliesNumber of Families with PHEO/Number of Total FamiliesNumber of Families with hyperPTH/Number of Total FamiliesNumber of Families with Other Diseases/NUMBER of Total Families
V648I30/30/31/3 a
E768D70/70/71/7 b
V804M502/501/5012/50 a
S891A120/120/122/12 b,1/12 d
C609R10/10/11/1 b
C6111F10/11/10/1
C618R52/50/51/5 b
C618S51/50/50/5
C620R41/40/41/4 a
C620S40/40/41/4 a
C620Y20/20/21/2 b, 1/2 a
C630Y30/31/31/3 c, a
C634F33/30/31/3 c
C634G42/40/41/4 c
C634R2013/203/202/20 c
C634S11/10/10/1
C634Y1511/153/151/15 c
C634W22/20/20/2
M918T154/150/1515/15
PHEO: pheochromocytoma; hyperPTH: hyperparathyroidism; a PTC; b Hirschprung’s disease; c Lichen cutaneous amyloidosic; d paraganglioma.

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Back to TopTop