Next Article in Journal
Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)
Previous Article in Journal
Characterization, Expression, and Interaction Analyses of OsMORF Gene Family in Rice
Previous Article in Special Issue
Temporal Dynamics of DNA Methylation Patterns in Response to Rearing Juvenile Steelhead (Oncorhynchus mykiss) in a Hatchery versus Simulated Stream Environment
Open AccessArticle

Histone Methylation Participates in Gene Expression Control during the Early Development of the Pacific Oyster Crassostrea gigas

1
Unité de Formation et de Recherches (UFR) des sciences, Université de Caen Normandie, 14032 Caen CEDEX, France
2
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), FRE2030, Muséum National d’Histoire Naturelle (MNHN), Centre National de la Recherche Scientifique (CNRS), Institut de Recherche et Développement (IRD), Sorbonne Université (SU), Université de Caen Normandie (UCN), Université des Antilles (UA), 75231 Paris CEDEX, France
3
Coastal Ecology Section, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Wadden Sea Station Sylt, 25992 List, Germany
4
Centre de Microscopie Appliquée à la Biologie, SF 4206 Interaction Cellule-Organisme-Environnement (ICORE), Université de Caen Normandie, Esplanade de la paix, 14032 Caen CEDEX, France
*
Author to whom correspondence should be addressed.
Genes 2019, 10(9), 695; https://doi.org/10.3390/genes10090695
Received: 2 August 2019 / Revised: 30 August 2019 / Accepted: 6 September 2019 / Published: 10 September 2019
(This article belongs to the Special Issue Epigenetics and Adaptation)
Histone methylation patterns are important epigenetic regulators of mammalian development, notably through stem cell identity maintenance by chromatin remodeling and transcriptional control of pluripotency genes. But, the implications of histone marks are poorly understood in distant groups outside vertebrates and ecdysozoan models. However, the development of the Pacific oyster Crassostrea gigas is under the strong epigenetic influence of DNA methylation, and Jumonji histone-demethylase orthologues are highly expressed during C. gigas early life. This suggests a physiological relevance of histone methylation regulation in oyster development, raising the question of functional conservation of this epigenetic pathway in lophotrochozoan. Quantification of histone methylation using fluorescent ELISAs during oyster early life indicated significant variations in monomethyl histone H3 lysine 4 (H3K4me), an overall decrease in H3K9 mono- and tri-methylations, and in H3K36 methylations, respectively, whereas no significant modification could be detected in H3K27 methylation. Early in vivo treatment with the JmjC-specific inhibitor Methylstat induced hypermethylation of all the examined histone H3 lysines and developmental alterations as revealed by scanning electronic microscopy. Using microarrays, we identified 376 genes that were differentially expressed under methylstat treatment, which expression patterns could discriminate between samples as indicated by principal component analysis. Furthermore, Gene Ontology revealed that these genes were related to processes potentially important for embryonic stages such as binding, cell differentiation and development. These results suggest an important physiological significance of histone methylation in the oyster embryonic and larval life, providing, to our knowledge, the first insights into epigenetic regulation by histone methylation in lophotrochozoan development. View Full-Text
Keywords: epigenetics; histone modifications; methylstat; embryos; mollusk; H3K4; H3K9; H3K27; H3K36 epigenetics; histone modifications; methylstat; embryos; mollusk; H3K4; H3K9; H3K27; H3K36
Show Figures

Figure 1

MDPI and ACS Style

Alexandre, F.; Lorane, L.; Aude, J.; Didier, G.; Pascal, F.; Guillaume, R. Histone Methylation Participates in Gene Expression Control during the Early Development of the Pacific Oyster Crassostrea gigas. Genes 2019, 10, 695.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop