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Open AccessArticle

The Duplicated Y-specific amhy Gene Is Conserved and Linked to Maleness in Silversides of the Genus Odontesthes

1
Unidade de Pesquisa e Desenvolvimento de Campos do Jordão, Sao Paulo Fisheries Institue, APTA/SAA, Campos do Jordão 12460-000, Brazil
2
Instituto Tecnológico de Chascomús (Consejo Nacional de Investigaciones Científicasy Técnicas—Universidad Nacional de San Martin), Chascomús 7130, Argentina
3
Instituto de Limnología “Dr. Raúl A. Ringuelet” (ILPLA) (Consejo Nacional de Investigaciones Científicasy Técnicas—Universidad Nacional de La Plata), La Plata 1900, Argentina
4
Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan
5
School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316002, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(9), 679; https://doi.org/10.3390/genes10090679
Received: 15 August 2019 / Revised: 3 September 2019 / Accepted: 3 September 2019 / Published: 5 September 2019
Sex-determining genes have been successively isolated in several teleosts. In Odontesthes hatcheri and O. bonariensis, the amhy gene has been identified as a master sex-determining gene. However, whether this gene is conserved along related species is still unknown. In this study, the presence of amhy and its association with phenotypic sex was analyzed in 10 species of Odontesthes genus. The primer sets from O. hatcheri that amplify both amhs successfully generated fragments that correspond to amha and amhy in all species. The full sequences of amhy and amha isolated for four key species revealed higher identity values among presumptive amhy, including the 0.5 Kbp insertion in the third intron and amhy-specific insertions/deletions. Amha was present in all specimens, regardless of species and sex, whereas amhy was amplified in most but not all phenotypic males. Complete association between amhy-homologue with maleness was found in O. argentinensis, O. incisa, O. mauleanum, O. perugiae, O. piquava, O. regia, and O. smitti, whereas O. humensis, O. mirinensis, and O. nigricans showed varied degrees of phenotypic/genotypic sex mismatch. The conservation of amhy gene in Odontesthes provide an interesting framework to study the evolution and the ecological interactions of genotypic and environmental sex determination in this group. View Full-Text
Keywords: Temperature-dependent sex determination (TSD); Genotypic sex determination (GSD); pejerrey; sex determination Temperature-dependent sex determination (TSD); Genotypic sex determination (GSD); pejerrey; sex determination
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Hattori, R.S.; Somoza, G.M.; Fernandino, J.I.; Colautti, D.C.; Miyoshi, K.; Gong, Z.; Yamamoto, Y.; Strüssmann, C.A. The Duplicated Y-specific amhy Gene Is Conserved and Linked to Maleness in Silversides of the Genus Odontesthes. Genes 2019, 10, 679.

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