DNA Testing Reveals the Putative Identity of JB55, a 19th Century Vampire Buried in Griswold, Connecticut
Abstract
:1. Introduction
2. Materials and Methods
2.1. Contamination Prevention
2.2. DNA Extraction
2.3. DNA Repair and Purification
2.4. DNA Quantification
2.5. Precision ID Ancestry SNP Panel
2.6. Y-STR Typing and Y-haplogroup Prediction
3. Results
3.1. DNA Quantification
3.2. Ancestry Estimation from Precision ID Ancestry SNP Panel
3.3. Y-STR Analysis
3.3.1. Yfiler Y-STR Typing
3.3.2. Y Haplogroup Estimation and Surname Prediction
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A. Illumina Sequencing of Yfiler Amplification Product
References
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Sample ID | Autosomal DNA Concentration (99 bp target) (ng/µL) | Y-Chromosomal DNA Concentration (133 bp target) (ng/µL) | Autosomal: Y-Chromosomal DNA Ratio |
---|---|---|---|
JB55-1 | 0.2009 | 0.0871 | 2.3072 |
RB-1 | 0 | 0 | 0 |
JB55-2 | 0.1735 | 0.0802 | 2.1624 |
RB-2 | 0 | 0 | 0 |
Locus | JB55-1 Allele(s) | JB55-2 Allele(s) |
---|---|---|
DYS19 | 15 | 15 |
DYS385 | 11,13 | 11,13 |
DYS389I | 13 | 13 |
DYS389II | 29 | 29 |
DYS390 | 23 | 23 |
DYS391 | 11 | 11 |
DYS392 | 13 | 13 |
DYS393 | 13 | 13 |
DYS437 | 15 | 15 |
DYS438 | 12 | No Data |
DYS439 | 12 | 12 |
DYS448 | 19 | 19 |
DYS456 | 15 | 15 |
DYS458 | 17 | 17 |
DYS635 | 23 | 23 |
Y GATA H4 | 12 | 12 |
JB55-1 | JB55-2 | ||||
---|---|---|---|---|---|
Allele | Read Count | % of Total Reads | Allele | Read Count | % of Total Reads |
12 | 12,343 | 96.3% | 12 | 8 | 88.9% |
11 | 386 | 3.0% | 13 | 1 | 11.1% |
Other | 87 | 0.7% | Other | 0 | 0% |
Total | 12,816 | 100% | Total | 9 | 100% |
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Daniels-Higginbotham, J.; Gorden, E.M.; Farmer, S.K.; Spatola, B.; Damann, F.; Bellantoni, N.; Gagnon, K.S.; de la Puente, M.; Xavier, C.; Walsh, S.; et al. DNA Testing Reveals the Putative Identity of JB55, a 19th Century Vampire Buried in Griswold, Connecticut. Genes 2019, 10, 636. https://doi.org/10.3390/genes10090636
Daniels-Higginbotham J, Gorden EM, Farmer SK, Spatola B, Damann F, Bellantoni N, Gagnon KS, de la Puente M, Xavier C, Walsh S, et al. DNA Testing Reveals the Putative Identity of JB55, a 19th Century Vampire Buried in Griswold, Connecticut. Genes. 2019; 10(9):636. https://doi.org/10.3390/genes10090636
Chicago/Turabian StyleDaniels-Higginbotham, Jennifer, Erin M. Gorden, Stephanie K. Farmer, Brian Spatola, Franklin Damann, Nicholas Bellantoni, Katie S. Gagnon, Maria de la Puente, Catarina Xavier, Susan Walsh, and et al. 2019. "DNA Testing Reveals the Putative Identity of JB55, a 19th Century Vampire Buried in Griswold, Connecticut" Genes 10, no. 9: 636. https://doi.org/10.3390/genes10090636
APA StyleDaniels-Higginbotham, J., Gorden, E. M., Farmer, S. K., Spatola, B., Damann, F., Bellantoni, N., Gagnon, K. S., de la Puente, M., Xavier, C., Walsh, S., Parson, W., McMahon, T. P., & Marshall, C. (2019). DNA Testing Reveals the Putative Identity of JB55, a 19th Century Vampire Buried in Griswold, Connecticut. Genes, 10(9), 636. https://doi.org/10.3390/genes10090636