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Open AccessArticle

Natural Root Cellular Variation in Responses to Osmotic Stress in Arabidopsis thaliana Accessions

1
Plant Molecular Genetic, Epigenetic, Development and Evolution laboratory. Ecology Institute. National Autonomous University of Mexico. 3er Circuito Ext. Junto a J. Botánico, Ciudad Universitaria. UNAM, Mexico City 04510, Mexico
2
Biological Science postgraduate, National Autonomous University of Mexico, Av. Universidad 3000, Coyoacán, Mexico City 04510, Mexico
3
Biochemical Science postgraduate, National Autonomous University of Mexico, Av. Universidad 3000, Coyoacán, Mexico City 04510, Mexico
4
Molecular Biology Center Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, Cantoblanco, 28049 Madrid, Spain
5
Botanical Garden, Biology Institute, National Autonomous University of Mexico, Mexico City 04510, Mexico
6
Complexity Science Center (C3), National Autonomous University of Mexico , Mexico City 04510, Mexico
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the article.
Genes 2019, 10(12), 983; https://doi.org/10.3390/genes10120983
Received: 25 October 2019 / Revised: 21 November 2019 / Accepted: 22 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Novel Insights into the Genetics of Root Development (2019))
Arabidopsis naturally occurring populations have allowed for the identification of considerable genetic variation remodeled by adaptation to different environments and stress conditions. Water is a key resource that limits plant growth, and its availability is initially sensed by root tissues. The root’s ability to adjust its physiology and morphology under water deficit makes this organ a useful model to understand how plants respond to water stress. Here, we used hyperosmotic shock stress treatments in different Arabidopsis accessions to analyze the root cell morphological responses. We found that osmotic stress conditions reduced root growth and root apical meristem (RAM) size, promoting premature cell differentiation without affecting the stem cell niche morphology. This phenotype was accompanied by a cluster of small epidermal and cortex cells with radial expansion and root hairs at the transition to the elongation zone. We also found this radial expansion with root hairs when plants are grown under hypoosmotic conditions. Finally, root growth was less affected by osmotic stress in the Sg-2 accession followed by Ws, Cvi-0, and Col-0; however, after a strong osmotic stress, Sg-2 and Cvi-0 were the most resilience accessions. The sensitivity differences among these accessions were not explained by stress-related gene expression. This work provides new cellular insights on the Arabidopsis root phenotypic variability and plasticity to osmotic stress.
Keywords: osmotic stress; Arabidopsis accessions; root morphology; plasticity; natural variation osmotic stress; Arabidopsis accessions; root morphology; plasticity; natural variation
MDPI and ACS Style

Cajero-Sanchez, W.; Aceves-Garcia, P.; Fernández-Marcos, M.; Gutiérrez, C.; Rosas, U.; García-Ponce, B.; Álvarez-Buylla, E.R.; Sánchez, M.P.; Garay-Arroyo, A. Natural Root Cellular Variation in Responses to Osmotic Stress in Arabidopsis thaliana Accessions. Genes 2019, 10, 983.

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