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Open AccessArticle

Testosterone Degradative Pathway of Novosphingobium tardaugens

Department of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas, Agencia Estatal Consejo Superior de Investigaciones Científicas. Ramiro de Maeztu 9, 28040 Madrid, Spain
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Genes 2019, 10(11), 871; https://doi.org/10.3390/genes10110871
Received: 4 September 2019 / Revised: 16 October 2019 / Accepted: 28 October 2019 / Published: 31 October 2019
(This article belongs to the Special Issue Genetics of Biodegradation and Bioremediation)
In this work, we have shown that Novosphingobium tardaugens NBRC 16725 (strain ARI-1), a bacterial strain that was isolated due to its capacity to mineralize the estrogenic endocrine compound 17β-estradiol, is also able to mineralize testosterone, the androgenic endocrine compound. Using in silico analysis, we predicted a new putative steroid degradation (SD) gene cluster in strain ARI-1, which resembles genes involved in testosterone degradation in Comamonas testosteroni and other testosterone degrading bacteria like Actinobacteria (like Rhodococcus and Mycobacteria genera) although with significant differences in gene organization. A whole transcriptomic analysis of N. tardaugens revealed that testosterone produces a limited induction of the genes of the SD cluster that show a high basal expression in its absence. The 3β/17β-hydroxysteroid dehydrogenase involved in the first metabolic step of testosterone degradation was identified by using genetic and biochemical approaches. The construction of knockout mutant strains in the genes of the SD cluster together with in silico analyses suggests the existence of gene redundancy in the genome of N. tardaugens. This work will expand the knowledge about the metabolic pathways and biotransformation capabilities of a Gram-negative bacterium that could become a new model system in the bacterial steroid degradation field. View Full-Text
Keywords: testosterone; steroid; catabolism; transcriptomic; biodegradation testosterone; steroid; catabolism; transcriptomic; biodegradation
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Ibero, J.; Galán, B.; Díaz, E.; García, J.L. Testosterone Degradative Pathway of Novosphingobium tardaugens. Genes 2019, 10, 871.

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