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Open AccessArticle

Integration of Quantitative Trait Loci Mapping and Expression Profiling Analysis to Identify Genes Potentially Involved in Ramie Fiber Lignin Biosynthesis

1
College of Biological and Environmental Engineering, Changsha University, Changsha 410003, China
2
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
3
College of biological and environmental sciences, Hunan University of Arts and Science, Changde 410128, China
4
Laboratory of ramie, Yichun Institute of Agricultural Sciences, Yichun 336000, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(11), 842; https://doi.org/10.3390/genes10110842
Received: 15 October 2019 / Accepted: 23 October 2019 / Published: 24 October 2019
(This article belongs to the Section Plant Genetics and Genomics)
Ramie fibers, one of the most important natural fibers in China, are mainly composed of lignin, cellulose, and hemicellulose. As the high lignin content in the fibers results in a prickly texture, the lignin content is deemed to be an important trait of the fiber quality. In this study, the genetic basis of the fiber lignin content was evaluated, resulting in the identification of five quantitative trait loci (QTLs). Three genes, whole_GLEAN_10021050, whole_GLEAN_10026962, and whole_GLEAN_10009464 that were identified on the QTL regions of qLC7, qLC10, and qLC13, respectively, were found to be homologs of the Arabidopsis lignin biosynthetic genes. Moreover, all three genes displayed differential expression in the barks located in the top and middle parts of the stem, where lignin was not being synthesized and where it was being biosynthesized, respectively. Sequence comparison found that these three genes had wide variations in their coding sequences (CDSs) and putative promoter regions between the two parents, especially the MYB gene whole_GLEAN_10021050, whose protein had insertions/deletions of five amino acids and substitutions of two amino acids in the conserved domain. This evidence indicates that these three genes are potentially involved in lignin biosynthesis in ramie fibers. The QTLs identified from this study provide a basis for the improvement of lignin content and fiber quality in ramie breeding. The characterization of the three candidate genes here will be helpful for the future clarification of their functions in ramie. View Full-Text
Keywords: ramie; lignin content; QTL; gene expression; sequence variation ramie; lignin content; QTL; gene expression; sequence variation
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Chen, J.; Rao, J.; Wang, Y.; Zeng, Z.; Liu, F.; Tang, Y.; Chen, X.; Liu, C.; Liu, T. Integration of Quantitative Trait Loci Mapping and Expression Profiling Analysis to Identify Genes Potentially Involved in Ramie Fiber Lignin Biosynthesis. Genes 2019, 10, 842.

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