Spatial Transcriptomic and miRNA Analyses Revealed Genes Involved in the Mesometrial-Biased Implantation in Pigs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples Collection
2.2. Histological Analysis
2.3. RNA-seq and miRNA-seq Data Analysis
2.4. Real-Time Polymerase Chain Reaction (RT-PCR) for Genes and miRNAs
2.5. Dual Luciferase Reporter Assays
3. Results
3.1. Analysis of the Histological Features of the Whole Cross-Sectional View of the Uterus during Implantation in Pigs
3.2. Gene Expression Patterns before and after Conceptus Attachment Differed between the Mesometrial and Anti-Mesometrial Endometrium in the Uterus
3.3. Endometrial Genes Exhibit Spatial Expression Patterns in the Uterus
3.4. Identification of Spatiotemporal Endometrial miRNA Expression Profiles in the Uterus during Implantation
3.5. Validation of the Predicted Interaction of miRNA-Target Pairs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Huang, J.; Yang, Y.; Tian, M.; Deng, D.; Yu, M. Spatial Transcriptomic and miRNA Analyses Revealed Genes Involved in the Mesometrial-Biased Implantation in Pigs. Genes 2019, 10, 808. https://doi.org/10.3390/genes10100808
Huang J, Yang Y, Tian M, Deng D, Yu M. Spatial Transcriptomic and miRNA Analyses Revealed Genes Involved in the Mesometrial-Biased Implantation in Pigs. Genes. 2019; 10(10):808. https://doi.org/10.3390/genes10100808
Chicago/Turabian StyleHuang, Ji, Yifen Yang, Miao Tian, Dadong Deng, and Mei Yu. 2019. "Spatial Transcriptomic and miRNA Analyses Revealed Genes Involved in the Mesometrial-Biased Implantation in Pigs" Genes 10, no. 10: 808. https://doi.org/10.3390/genes10100808