Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly, Liriomyza trifolii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Insects of Different Developmental Stages
2.2. Temperature Treatments
2.3. RNA Isolation and Cloning Experiments
2.4. Reverse Transcription Quantitative PCR
2.5. Sequence Alignment of sHSPs and Data Analysis
3. Results
3.1. Cloning, Characterization and Phylogenetic Analysis of Three sHSP Genes from Liriomyza trifolii
3.2. Expression of Three LtsHSPs in Response to Temperature Treatments
3.3. Expression of Three LtsHSPs at the Developmental Stages
3.4. Comparative Characteristics and Expression Pattern of Five LtsHSPs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Name of Genes | Molecular Weight | Isoelectric Point | Developmental Stages (Highest Expression) | High Temperature (Tmax) | Low Temperature (Tmax) |
---|---|---|---|---|---|
LtHSP19.5 | 19.46 kDa | 5.56 | prepupae | 40 °C | −17.5 °C |
LtHSP20.8 | 20.89 kDa | 6.71 | two-day-old pupae | 40 °C | −17.5 °C |
LtHSP21.7b | 21.72 kDa | 6.07 | two-day-old pupae | 40 °C | −17.5 °C |
LtHSP21.3 | 21.23 kDa * | 6.38 * | prepupae | 40 °C * | −17.5 °C * |
LtHSP21.7 | 21.66 kDa * | 7.03 * | prepupae * | 42.5 °C | −17.5 °C |
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Chang, Y.-W.; Zhang, X.-X.; Lu, M.-X.; Du, Y.-Z.; Zhu-Salzman, K. Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly, Liriomyza trifolii. Genes 2019, 10, 775. https://doi.org/10.3390/genes10100775
Chang Y-W, Zhang X-X, Lu M-X, Du Y-Z, Zhu-Salzman K. Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly, Liriomyza trifolii. Genes. 2019; 10(10):775. https://doi.org/10.3390/genes10100775
Chicago/Turabian StyleChang, Ya-Wen, Xiao-Xiang Zhang, Ming-Xing Lu, Yu-Zhou Du, and Keyan Zhu-Salzman. 2019. "Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly, Liriomyza trifolii" Genes 10, no. 10: 775. https://doi.org/10.3390/genes10100775