Next Article in Journal
Patterns and Constraints in the Evolution of Sperm Individualization Genes in Insects, with an Emphasis on Beetles
Previous Article in Journal
Comparison of the Whole-Plastome Sequence between the Bonin Islands Endemic Rubus boninensis and Its Close Relative, Rubus trifidus (Rosaceae), in the Southern Korean Peninsula
Open AccessArticle

Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly, Liriomyza trifolii

1
College of Horticulture and Plant Protection, Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China
2
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education, Yangzhou University, Yangzhou 225009, China
3
Department of Entomology, Texas A&M University, College Station, TX 77843, USA
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(10), 775; https://doi.org/10.3390/genes10100775
Received: 23 June 2019 / Revised: 26 September 2019 / Accepted: 27 September 2019 / Published: 3 October 2019
(This article belongs to the Section Molecular Genetics and Genomics)
Small heat shock proteins (sHSPs) comprise numerous proteins with diverse structure and function. As molecular chaperones, they play essential roles in various biological processes, especially under thermal stresses. In this study, we identified three sHSP-encoding genes, LtHSP19.5, LtHSP20.8 and LtHSP21.7b from Liriomyza trifolii, an important insect pest of ornamental and vegetable crops worldwide. Putative proteins encoded by these genes all contain a conserved α-crystallin domain that is typical of the sHSP family. Their expression patterns during temperature stresses and at different insect development stages were studied by reverse-transcription quantitative PCR (RT-qPCR). In addition, the expression patterns were compared with those of LtHSP21.3 and LtHSP21.7, two previously published sHSPs. When pupae were exposed to temperatures ranging from −20 to 45 °C for 1 h, all LtsHSPs were strongly induced by either heat or cold stresses, but the magnitude was lower under the low temperature range than high temperatures. Developmentally regulated differential expression was also detected, with pupae and prepupae featuring the highest expression of sHSPs. Results suggest that LtsHSPs play a role in the development of the invasive leaf miner fly and may facilitate insect adaptation to climate change. View Full-Text
Keywords: Liriomyza trifolii; small heat shock protein; development stage; thermal stress; expression pattern Liriomyza trifolii; small heat shock protein; development stage; thermal stress; expression pattern
Show Figures

Figure 1

MDPI and ACS Style

Chang, Y.-W.; Zhang, X.-X.; Lu, M.-X.; Du, Y.-Z.; Zhu-Salzman, K. Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly, Liriomyza trifolii. Genes 2019, 10, 775.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop