In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Growth Conditions
2.2. Resistance to Gastric Conditions
2.3. The In Vitro Epithelial Barrier Model
2.4. In Vitro Immunomodulation Assay
2.5. Murine Model of 2,4,6-Trinitrobenzenesulfonic Acid (TNBS)-Induced Colitis
2.6. Quantification of Fecal Lipocalin 2 (Lcn-2)
2.7. Real-Time Quantitative PCR (qRT-PCR)
2.8. Immunofluorescence Staining and Confocal Analysis
2.9. DNA Extraction from Stool and P. distasonis Quantification by qPCR
2.10. Preparation of Bacteria-Primed Bone Marrow Derived Dendritic Cells (BMDCs) and Co-Culture with Naive CD4+ T Cells
2.11. Statistical Analysis
3. Results
3.1. P. distasonis Strains Displayed Different Ability to Restore the H2O2-Induced Disruption of the Epithelial Barrier
3.2. P. distasonis PF-BaE5 and PF-BaE11 Exhibit the Best Anti-Inflammatory In Vitro Profile
3.3. Strains Survive Differently to Gastric Condition
3.4. P. distasonis PF-BaE5, PF-BaE11 and AS93 Were the Most Potent Strains to Counteract Inflammation in a Murine Model of TNBS-Induced Colitis
3.5. P. distasonis PF-BaE5 and PF-BaE11 Led to Immature Bone Marrow Dendritic Cells (BMDCs) In Vitro and Regulatory T Lymphocytes When Co-Cultured with Naïve CD4+ T Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Strains Designation | Species | Growth Medium | Origin |
---|---|---|---|
AS93 | Parabacteroides distasonis | BHIS | Healthy adult feces |
AS23 | Parabacteroides distasonis | BHIS | Healthy adult feces |
PF-BaE5 | Parabacteroides distasonis | BHIS | Newborn fecal samples |
PF-BaE7 | Parabacteroides distasonis | BHIS | Newborn fecal samples |
PF-BaE11 | Parabacteroides distasonis | BHIS | Newborn fecal samples |
Strains | Improvement of Epithelial Barrier | IL-10 Induction | Anti-Inflammatory Profile (Il-10/IL-12) | Tolerance to Gastric Conditions (120 min) | In Vivo Protective Capacity |
---|---|---|---|---|---|
P. distasonis AS23 | ± | +++ | + | ++ | − |
P. distasonis AS93 | +++ | +++ | + | ± | ++ |
P. distasonis PF-BaE5 | ++ | +++ | ++ | ± | +++ |
P. distasonis PF-BaE7 | ± | +++ | + | ++ | − |
P. distasonis PF-BaE11 | +++ | +++ | ++ | ± | +++ |
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Cuffaro, B.; Assohoun, A.L.W.; Boutillier, D.; Súkeníková, L.; Desramaut, J.; Boudebbouze, S.; Salomé-Desnoulez, S.; Hrdý, J.; Waligora-Dupriet, A.-J.; Maguin, E.; et al. In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice. Cells 2020, 9, 2104. https://doi.org/10.3390/cells9092104
Cuffaro B, Assohoun ALW, Boutillier D, Súkeníková L, Desramaut J, Boudebbouze S, Salomé-Desnoulez S, Hrdý J, Waligora-Dupriet A-J, Maguin E, et al. In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice. Cells. 2020; 9(9):2104. https://doi.org/10.3390/cells9092104
Chicago/Turabian StyleCuffaro, Bernardo, Aka L. W. Assohoun, Denise Boutillier, Lenka Súkeníková, Jérémy Desramaut, Samira Boudebbouze, Sophie Salomé-Desnoulez, Jiří Hrdý, Anne-Judith Waligora-Dupriet, Emmanuelle Maguin, and et al. 2020. "In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice" Cells 9, no. 9: 2104. https://doi.org/10.3390/cells9092104