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Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts

1
Department of Cranial Maxillofacial Plastic Surgery, University Clinic Leipzig, 04103 Leipzig, Germany
2
School of (EAST) Engineering, Arts, Science & Technology, University of Suffolk, Ipswich, Suffolk IP41QJ, UK
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Clinic for Hematology, Cell Therapy and Hemostaseology, University Hospital Leipzig, 04103 Leipzig, Germany
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Polyclinic for Dental Prosthetics and Material Sciences, University Hospital Leipzig, 04103 Leipzig, Germany
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Kensuke Yamauchi, Department of Oral & Maxillofacial Surgery, Tohoku University, Sendai 980-8577, Japan
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Leipzig Heart Center, 04289 Leipzig, Germany
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Chonnam National University Hwasun Hospital, Hwasun-gun 58128, Korea
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Clinic for Dermatology, Venereology and Allergology, University Hospital Leipzig, 04103 Leipzig, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2020, 9(9), 2069; https://doi.org/10.3390/cells9092069
Received: 7 August 2020 / Revised: 4 September 2020 / Accepted: 6 September 2020 / Published: 10 September 2020
(This article belongs to the Section Stem Cells)
Background: Regenerative therapies based on autologous mesenchymal stem cells (MSC) as well as stem cells in general are still facing an unmet need for non-invasive sampling, availability, and scalability. The only known adult source of autologous MSCs permanently available with no pain, discomfort, or infection risk is the outer root sheath of the hair follicle (ORS). Methods: This study presents a non-invasively-based method for isolating and expanding MSCs from the ORS (MSCORS) by means of cell migration and expansion in air–liquid culture. Results: The method yielded 5 million cells of pure MSCORS cultured in 35 days, thereby superseding prior art methods of culturing MSCs from hair follicles. MSCORS features corresponded to the International Society for Cell Therapy characterization panel for MSCs: adherence to plastic, proliferation, colony forming, expression of MSC-markers, and adipo-, osteo-, and chondro-differentiation capacity. Additionally, MSCORS displayed facilitated random-oriented migration and high proliferation, pronounced marker expression, extended endothelial and smooth muscle differentiation capacity, as well as a paracrine immunomodulatory effect on monocytes. MSCORS matched or even exceeded control adipose-derived MSCs in most of the assessed qualities. Conclusions: MSCORS qualify for a variety of autologous regenerative treatments of chronic disorders and prophylactic cryopreservation for purposes of acute treatments in personalized medicine. View Full-Text
Keywords: mesenchymal stem cells; hair follicle; regenerative medicine; non-invasive; cell therapy; adipose tissue; differentiation mesenchymal stem cells; hair follicle; regenerative medicine; non-invasive; cell therapy; adipose tissue; differentiation
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MDPI and ACS Style

Li, H.; Masieri, F.F.; Schneider, M.; Kottek, T.; Hahnel, S.; Yamauchi, K.; Obradović, D.; Seon, J.-K.; Yun, S.J.; Ferrer, R.A.; Franz, S.; Simon, J.-C.; Lethaus, B.; Savković, V. Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts. Cells 2020, 9, 2069. https://doi.org/10.3390/cells9092069

AMA Style

Li H, Masieri FF, Schneider M, Kottek T, Hahnel S, Yamauchi K, Obradović D, Seon J-K, Yun SJ, Ferrer RA, Franz S, Simon J-C, Lethaus B, Savković V. Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts. Cells. 2020; 9(9):2069. https://doi.org/10.3390/cells9092069

Chicago/Turabian Style

Li, Hanluo, Federica F. Masieri, Marie Schneider, Tina Kottek, Sebastian Hahnel, Kensuke Yamauchi, Danilo Obradović, Jong-Keun Seon, Sook J. Yun, Rubén A. Ferrer, Sandra Franz, Jan-Christoph Simon, Bernd Lethaus, and Vuk Savković. 2020. "Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts" Cells 9, no. 9: 2069. https://doi.org/10.3390/cells9092069

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