Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Culture of MSCORS
2.2. Isolation and Culture of Adipose Derived MSCs (ADMSCs)
2.3. Determination of Cell Count and Cell Mitochondrial Activity
2.4. Determination of Cell Movement: Live Cell Imaging Time Lapse
2.5. Determination of Gene Expression: Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
2.6. Determination of Cell Surface Marker: Fluorescence-Activated Cell Sorting (FACS)
2.7. Determination of Cell Surface Marker and Differentiation Markers: Immunofluorescence Staining
2.8. Osteogenic Differentiation
2.9. Chondrogenic Differentiation
2.10. Adipogenic Differentiation
2.11. Endothelial Differentiation
2.12. Smooth Muscle Differentiation
2.13. Determination of Cell Immunomodulatory Effects
2.14. Statistical Analysis
3. Results
3.1. MSCORS Isolation by Migration and Adherent Culture
3.2. Cell Proliferation and Mitochondrial Activity
3.3. Cell Motility and Migration Parameters
3.4. Gene Expression
3.5. MSC Surface Marker Expression
3.6. Protein Expression and Subcellular Distribution
3.7. Differentiation Potentials
3.8. Immunomodulatory Effects of MSCORS vs. ADMSC
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Number of Hair Follicles | Hair Weight (mg) | Cell Yield in P0 (Million Cells) | Cell Yield in P1 (Million Cells) | Cell Yield in P2 (Million Cells) |
---|---|---|---|---|
60 | 21.45 ± 12.17 | 5.00 ± 2.68 | 21.90 ± 9.46 | 50.31 ± 27.79 |
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Li, H.; Masieri, F.F.; Schneider, M.; Kottek, T.; Hahnel, S.; Yamauchi, K.; Obradović, D.; Seon, J.-K.; Yun, S.J.; Ferrer, R.A.; et al. Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts. Cells 2020, 9, 2069. https://doi.org/10.3390/cells9092069
Li H, Masieri FF, Schneider M, Kottek T, Hahnel S, Yamauchi K, Obradović D, Seon J-K, Yun SJ, Ferrer RA, et al. Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts. Cells. 2020; 9(9):2069. https://doi.org/10.3390/cells9092069
Chicago/Turabian StyleLi, Hanluo, Federica Francesca Masieri, Marie Schneider, Tina Kottek, Sebastian Hahnel, Kensuke Yamauchi, Danilo Obradović, Jong-Keun Seon, Sook Jung Yun, Rubén A. Ferrer, and et al. 2020. "Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Follicle Are Obtainable by Migration from Plucked Hair Follicles and Expandable in Scalable Amounts" Cells 9, no. 9: 2069. https://doi.org/10.3390/cells9092069