Next Article in Journal
A Role for Caenorhabditis elegans COMPASS in Germline Chromatin Organization
Next Article in Special Issue
The Rationale of Neprilysin Inhibition in Prevention of Myocardial Ischemia-Reperfusion Injury during ST-Elevation Myocardial Infarction
Previous Article in Journal
Rebuilding Tendons: A Concise Review on the Potential of Dermal Fibroblasts
Article

HPLC-MS/MS Shows That the Cellular Uptake of All-Trans-Retinoic Acid under Hypoxia Is Downregulated by the Novel Active Agent 5-Methoxyleoligin

1
Institute of Analytical Chemistry, Johannes Kepler University Linz, 4040 Linz, Austria
2
Division of Pathophysiology, Institute of Physiology and Pathophysiology, Medical Faculty, Johannes Kepler University Linz, 4020 Linz, Austria
*
Author to whom correspondence should be addressed.
Cells 2020, 9(9), 2048; https://doi.org/10.3390/cells9092048
Received: 30 July 2020 / Revised: 28 August 2020 / Accepted: 7 September 2020 / Published: 8 September 2020
All-trans-retinoic acid (atRA) is the essential derivative of vitamin A and is of interest due to its various biological key functions. As shown in the recent literature, atRA also plays a role in the failing heart during myocardial infarction, the leading cause of death globally. To date insufficient mechanistic information has been available on related hypoxia-induced cell damage and reperfusion injuries. However, it has been demonstrated that a reduction in cellular atRA uptake abrogates hypoxia-mediated cell and tissue damage, which may offer a new route for intervention. Consequently, in this study, the effect of the novel cardio-protective compound 5-methoxyleoligin (5ML) on cellular atRA uptake was tested in human umbilical-vein endothelial cells (HUVECs). For this purpose, a high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed to assess intra-cellular levels of the active substance and corresponding levels of vitamin A and its derivatives, including potential cis/trans isomers. This work also focused on light-induced isomerization and the stability of biological sample material to ensure sample integrity and avoid biased conclusions. This study provides evidence of the inhibitory effect of 5ML on cellular atRA uptake, a promising step toward a novel therapy for myocardial infarction. View Full-Text
Keywords: myocardial infarction; all-trans-retinoic acid; 5-methoxyleoligin; HPLC-MS/MS; photoisomerization myocardial infarction; all-trans-retinoic acid; 5-methoxyleoligin; HPLC-MS/MS; photoisomerization
Show Figures

Figure 1

MDPI and ACS Style

Guntner, A.S.; Doppler, C.; Wechselberger, C.; Bernhard, D.; Buchberger, W. HPLC-MS/MS Shows That the Cellular Uptake of All-Trans-Retinoic Acid under Hypoxia Is Downregulated by the Novel Active Agent 5-Methoxyleoligin. Cells 2020, 9, 2048. https://doi.org/10.3390/cells9092048

AMA Style

Guntner AS, Doppler C, Wechselberger C, Bernhard D, Buchberger W. HPLC-MS/MS Shows That the Cellular Uptake of All-Trans-Retinoic Acid under Hypoxia Is Downregulated by the Novel Active Agent 5-Methoxyleoligin. Cells. 2020; 9(9):2048. https://doi.org/10.3390/cells9092048

Chicago/Turabian Style

Guntner, Armin S., Christian Doppler, Christian Wechselberger, David Bernhard, and Wolfgang Buchberger. 2020. "HPLC-MS/MS Shows That the Cellular Uptake of All-Trans-Retinoic Acid under Hypoxia Is Downregulated by the Novel Active Agent 5-Methoxyleoligin" Cells 9, no. 9: 2048. https://doi.org/10.3390/cells9092048

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop