Arabidopsis thaliana cbp80, c2h2, and flk Knockout Mutants Accumulate Increased Amounts of Circular RNAs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and RNA Extraction
2.2. Library Preparation and Sequencing
2.3. CircRNA Identification and Quantification
2.4. Transcript-Level Expression and Alternative Splicing Analysis
3. Results
3.1. Selection of the A. thaliana Knock-Out Mutants
3.2. CircRNA Identification in Wild-Type and Mutant Plants
3.3. CircRNAs in the cbp80 Mutant
3.4. CircRNAs in the c2h2 Mutant
3.5. CircRNAs in the flk Mutant
3.6. CircRNA Production as a Form of Alternative Splicing
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Philips, A.; Nowis, K.; Stelmaszczuk, M.; Podkowiński, J.; Handschuh, L.; Jackowiak, P.; Figlerowicz, M. Arabidopsis thaliana cbp80, c2h2, and flk Knockout Mutants Accumulate Increased Amounts of Circular RNAs. Cells 2020, 9, 1937. https://doi.org/10.3390/cells9091937
Philips A, Nowis K, Stelmaszczuk M, Podkowiński J, Handschuh L, Jackowiak P, Figlerowicz M. Arabidopsis thaliana cbp80, c2h2, and flk Knockout Mutants Accumulate Increased Amounts of Circular RNAs. Cells. 2020; 9(9):1937. https://doi.org/10.3390/cells9091937
Chicago/Turabian StylePhilips, Anna, Katarzyna Nowis, Michal Stelmaszczuk, Jan Podkowiński, Luiza Handschuh, Paulina Jackowiak, and Marek Figlerowicz. 2020. "Arabidopsis thaliana cbp80, c2h2, and flk Knockout Mutants Accumulate Increased Amounts of Circular RNAs" Cells 9, no. 9: 1937. https://doi.org/10.3390/cells9091937