Next Article in Journal
Marine Actinomycetes-Derived Secondary Metabolites Overcome TRAIL-Resistance via the Intrinsic Pathway through Downregulation of Survivin and XIAP
Next Article in Special Issue
Establishment of a Rapid Lesion-Controllable Retinal Degeneration Monkey Model for Preclinical Stem Cell Therapy
Previous Article in Journal
The RNA Methyltransferase NSUN2 and Its Potential Roles in Cancer
 
 
Article

Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells

by 1,†, 2,†, 2,‡ and 1,*,‡
1
Department of Cell Biology and Histology, University of Basque Country UPV/EHU, Leioa, 48940 Vizcaya, Spain
2
Department of Cell Biology and Human Anatomy, University of California Davis, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Co-Senior authors.
Cells 2020, 9(8), 1759; https://doi.org/10.3390/cells9081759
Received: 5 June 2020 / Revised: 13 July 2020 / Accepted: 15 July 2020 / Published: 22 July 2020
(This article belongs to the Special Issue Stem Cells in Retinal or Optic Nerve Disease and Development)
Retinal neurons, particularly retinal ganglion cells (RGCs), are susceptible to the degenerative damage caused by different inherited conditions and environmental insults, leading to irreversible vision loss and, ultimately, blindness. Numerous strategies are being tested in different models of degeneration to restore vision and, in recent years, stem cell technologies have offered novel avenues to obtain donor cells for replacement therapies. To date, stem cell–based transplantation in the retina has been attempted as treatment for photoreceptor degeneration, but the same tools could potentially be applied to other retinal cell types, including RGCs. However, RGC-like cells are not an abundant cell type in stem cell–derived cultures and, often, these cells degenerate over time in vitro. To overcome this limitation, we have taken advantage of the neuroprotective properties of Müller glia (one of the main glial cell types in the retina) and we have examined whether Müller glia and the factors they secrete could promote RGC-like cell survival in organoid cultures. Accordingly, stem cell-derived RGC-like cells were co-cultured with adult Müller cells or Müller cell-conditioned media was added to the cultures. Remarkably, RGC-like cell survival was substantially enhanced in both culture conditions, and we also observed a significant increase in their neurite length. Interestingly, Atoh7, a transcription factor required for RGC development, was up-regulated in stem cell-derived organoids exposed to conditioned media, suggesting that Müller cells may also enhance the survival of retinal progenitors and/or postmitotic precursor cells. In conclusion, Müller cells and the factors they release promote organoid-derived RGC-like cell survival, neuritogenesis, and possibly neuronal maturation. View Full-Text
Keywords: retinal ganglion cells; Müller glia; Stem cells; retinal organoids; neuroprotection; neuritogenesis retinal ganglion cells; Müller glia; Stem cells; retinal organoids; neuroprotection; neuritogenesis
Show Figures

Figure 1

MDPI and ACS Style

Pereiro, X.; Miltner, A.M.; La Torre, A.; Vecino, E. Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells. Cells 2020, 9, 1759. https://doi.org/10.3390/cells9081759

AMA Style

Pereiro X, Miltner AM, La Torre A, Vecino E. Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells. Cells. 2020; 9(8):1759. https://doi.org/10.3390/cells9081759

Chicago/Turabian Style

Pereiro, Xandra, Adam M. Miltner, Anna La Torre, and Elena Vecino. 2020. "Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells" Cells 9, no. 8: 1759. https://doi.org/10.3390/cells9081759

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop