Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues
Abstract
:1. Introduction
2. Materials and Methods
2.1. MCEV Isolation from Brain Tissue
2.2. SDS-PAGE Gel Electrophoresis
2.3. Nanoparticle Tracking Analysis
2.4. Transmission Electron Microscopy
2.5. Mass Spectrometry
2.6. Data Analysis and Protein Identification
3. Results
3.1. MCEVs Isolated Display the Characteristics of Small EVs
3.2. MCEVs Display a Unique Proteome Compared to Their respective TBs
3.3. Differentially Expressed Proteins Found in ALS MCEVs Compared to NC MCEVs
3.4. Differentially Expressed Proteins in ALS MCEVs Are Involved in Communication, Exhibit RNA-Binding Activity and Are Sequestered to Lysosomes and Exosomes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Vassileff, N.; Vella, L.J.; Rajapaksha, H.; Shambrook, M.; Kenari, A.N.; McLean, C.; Hill, A.F.; Cheng, L. Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues. Cells 2020, 9, 1709. https://doi.org/10.3390/cells9071709
Vassileff N, Vella LJ, Rajapaksha H, Shambrook M, Kenari AN, McLean C, Hill AF, Cheng L. Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues. Cells. 2020; 9(7):1709. https://doi.org/10.3390/cells9071709
Chicago/Turabian StyleVassileff, Natasha, Laura J. Vella, Harinda Rajapaksha, Mitch Shambrook, Amirmohammad Nasiri Kenari, Catriona McLean, Andrew F. Hill, and Lesley Cheng. 2020. "Revealing the Proteome of Motor Cortex Derived Extracellular Vesicles Isolated from Amyotrophic Lateral Sclerosis Human Postmortem Tissues" Cells 9, no. 7: 1709. https://doi.org/10.3390/cells9071709