Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmids
2.2. Cell Lines and Transfections
2.3. Immunofluorescence
2.4. Pulldown of Biotinylated Proteins
2.5. Mass Spectrometry
2.6. Data Analysis
2.7. Gene Ontology and Pathway Analysis
2.8. Screening for Nuclear Export Signals
3. Results
3.1. Subcellular Distribution of BioID Fusion Proteins and Biotinylation Induction
3.2. Identification of Known Proximal Interactors of NUP98-HOXA9 and SET-NUP214
3.3. Identification of Novel Proximal Interactors of NUP98-HOXA9
3.4. NUP98-HOXA9 Proximal Interactors are Enriched in Nuclear Export Signal (NES) Containing Proteins
3.5. Identification of Novel Proximal Interactors of SET-NUP214
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
Appendix A.1. Cloning of SET-NUP214 into the pcDNA3.1 MCS-BirA(R118G)-HA Vector
Appendix A.2. Incubation and Elution of Biotinylated Proteins from Streptavidin-Coated Magnetic Beads
Appendix A.3. Sample Injection and Mass Spectrometer Operation
Appendix A.4. Raw Data Analysis Using MaxQuant
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Protein | Gene | NHA9-BioID | F.C. | Ref. |
---|---|---|---|---|
Nucleoporin NUP98 | NUP98 | +/+ | −1.84 | [46] |
Chromosome region maintenance 1/Exportin 1 | CRM1/XPO1 | +/+ | −2.13 | [24,29] |
mRNA export factor 1 | RAE1 | +/+ | 6.40 | [46] |
Nuclear factor of activated T cells | NFAT5 | +/- | 24.81 | [24] |
Histone-lysine N-methyltransferase 2A/Mixed lineage leukemia 1 | KMT2A/ MLL1 | +/+ | −0.91 | [28] |
Host cell factor 1 | HCFC1 | +/+ | −1.86 | [27] |
Histone deacetylase 1 | HDAC1 | +/+ | −2.05 | [47] |
O-GlcNac transferase subunit p110 | OGT | -/- | [27] | |
CREB binding protein | CREBBP/p300 | -/- | [47,48] |
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Mendes, A.; Jühlen, R.; Bousbata, S.; Fahrenkrog, B. Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach. Cells 2020, 9, 1666. https://doi.org/10.3390/cells9071666
Mendes A, Jühlen R, Bousbata S, Fahrenkrog B. Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach. Cells. 2020; 9(7):1666. https://doi.org/10.3390/cells9071666
Chicago/Turabian StyleMendes, Adélia, Ramona Jühlen, Sabrina Bousbata, and Birthe Fahrenkrog. 2020. "Disclosing the Interactome of Leukemogenic NUP98-HOXA9 and SET-NUP214 Fusion Proteins Using a Proteomic Approach" Cells 9, no. 7: 1666. https://doi.org/10.3390/cells9071666