Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Western Blot Analysis
2.3. Live Confocal Microscopy and Analysis of Mitochondrial Morphology
2.4. Seahorse Analysis of Oxygen Consumption and Extracellular Acidification Rates
2.5. TUNEL Assay
2.6. Statistical Analysis
3. Results
3.1. Effects of HG and Drp1/Fis1 siRNAs on Fission Genes in RRECs
3.2. Effects of HG and Drp1/Fis1 siRNAs on Apoptotic Genes in RRECs
3.3. Drp1/Fis1 Downregulation Prevents Mitochondrial Fragmentation in RRECs
3.4. Reduced Mitochondrial Fission Prevents HG-Induced Apoptosis in RRECs
3.5. Inhibiting Mitochondrial Fission Gene Expression Improves Mitochondrial Respiration in RRECs
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Kim, D.; Sankaramoorthy, A.; Roy, S. Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells. Cells 2020, 9, 1662. https://doi.org/10.3390/cells9071662
Kim D, Sankaramoorthy A, Roy S. Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells. Cells. 2020; 9(7):1662. https://doi.org/10.3390/cells9071662
Chicago/Turabian StyleKim, Dongjoon, Aravind Sankaramoorthy, and Sayon Roy. 2020. "Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells" Cells 9, no. 7: 1662. https://doi.org/10.3390/cells9071662
APA StyleKim, D., Sankaramoorthy, A., & Roy, S. (2020). Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells. Cells, 9(7), 1662. https://doi.org/10.3390/cells9071662