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The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells

1
The School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD 4035, Australia
2
Saliva and Liquid Biopsy Translational Research Team, Institute of Health and Biomedical Innovation (IHBI), Queensland University of Technology (QUT), Kelvin Grove, QLD 4035, Australia
3
Translational Research Institute, Woolloongabba, QLD 4102 Australia
4
The School of Biomedical Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia
5
Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
*
Author to whom correspondence should be addressed.
Cells 2020, 9(6), 1465; https://doi.org/10.3390/cells9061465
Received: 7 May 2020 / Revised: 2 June 2020 / Accepted: 11 June 2020 / Published: 15 June 2020
(This article belongs to the Section Cellular Pathology)
Tumor tissue biopsy is often limited for non-small cell lung cancer (NSCLC) patients and alternative sources of tumoral information are desirable to determine molecular alterations such as anaplastic lymphoma kinase (ALK) rearrangements. Circulating tumor cells (CTCs) are an appealing component of liquid biopsies, which can be sampled serially over the course of treatment. In this study, we enrolled a cohort of ALK-positive (n = 8) and ALK-negative (n = 12) NSCLC patients, enriched for CTCs using spiral microfluidic technology and performed DNA fluorescent in situ hybridization (FISH) for ALK. CTCs were identified in 12/20 NSCLC patients ranging from 1 to 26 CTCs/7.5 mL blood. Our study revealed that 3D imaging of CTCs for ALK translocations captured a well-defined separation of 3′ and 5′ signals indicative of ALK translocations and overlapping 3′/5′ signal was easily resolved by imaging through the nuclear volume. This study provides proof-of-principle for the use of 3D DNA FISH in the determination of CTC ALK translocations in NSCLC. View Full-Text
Keywords: circulating tumor cells; anaplastic lymphoma kinase; non-small cell lung cancer circulating tumor cells; anaplastic lymphoma kinase; non-small cell lung cancer
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MDPI and ACS Style

Kulasinghe, A.; Lim, Y.; Kapeleris, J.; Warkiani, M.; O’Byrne, K.; Punyadeera, C. The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells. Cells 2020, 9, 1465. https://doi.org/10.3390/cells9061465

AMA Style

Kulasinghe A, Lim Y, Kapeleris J, Warkiani M, O’Byrne K, Punyadeera C. The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells. Cells. 2020; 9(6):1465. https://doi.org/10.3390/cells9061465

Chicago/Turabian Style

Kulasinghe, Arutha; Lim, Yenkai; Kapeleris, Joanna; Warkiani, Majid; O’Byrne, Ken; Punyadeera, Chamindie. 2020. "The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells" Cells 9, no. 6: 1465. https://doi.org/10.3390/cells9061465

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