Cuprizone Affects Hypothermia-Induced Neuroprotection and Enhanced Neuroblast Differentiation in the Gerbil Hippocampus after Ischemia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Experimental Groups and Treatments
2.3. Induction of Transient Forebrain Ischemia
2.4. Tissue Processing
2.5. Cresyl Violet Staining
2.6. Immunohistochemistry for Glial Fibrillary Acidic Protein (GFAP), Ionized Calcium-Binding Adapter Molecule 1 (Iba-1), MBP, Mac3, Ki67, and Doublecortin (DCX)
2.7. Enzyme Immunoassay for Cytokines
2.8. Semi-Quantification of Data
2.9. Statistical Analysis
3. Results
3.1. Effects of Cuprizone on Cell Survival after Ischemia in Normothermic and Hypothermic Gerbils
3.2. Effects of Cuprizone on Reactive Astrocytosis after Ischemia in Normothermic and Hypothermic Gerbils
3.3. Effects of Cuprizone on Reactive Microgliosis after Ischemia in Normothermic and Hypothermic Gerbils
3.4. Effects of Cuprizone on Macrophages after Ischemia in Normothermic and Hypothermic Gerbils
3.5. Effects of Cuprizone on MBP Expression after Ischemia in Normothermic and Hypothermic Gerbils
3.6. Effects of Cuprizone on IL-1β and TNF-α after Ischemia in Normothermic and Hypothermic Gerbils
3.7. Effects of Cuprizone on Proliferating Cells after Ischemia in Normothermic and Hypothermic Gerbils
3.8. Effects of Cuprizone on Differentiated Neuroblasts after Ischemia in Normothermic and Hypothermic Gerbils
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Kim, W.; Hahn, K.R.; Jung, H.Y.; Kwon, H.J.; Nam, S.M.; Kim, T.H.; Kim, J.W.; Yoo, D.Y.; Kim, D.W.; Choi, J.H.; et al. Cuprizone Affects Hypothermia-Induced Neuroprotection and Enhanced Neuroblast Differentiation in the Gerbil Hippocampus after Ischemia. Cells 2020, 9, 1438. https://doi.org/10.3390/cells9061438
Kim W, Hahn KR, Jung HY, Kwon HJ, Nam SM, Kim TH, Kim JW, Yoo DY, Kim DW, Choi JH, et al. Cuprizone Affects Hypothermia-Induced Neuroprotection and Enhanced Neuroblast Differentiation in the Gerbil Hippocampus after Ischemia. Cells. 2020; 9(6):1438. https://doi.org/10.3390/cells9061438
Chicago/Turabian StyleKim, Woosuk, Kyu Ri Hahn, Hyo Young Jung, Hyun Jung Kwon, Sung Min Nam, Tae Hyeong Kim, Jong Whi Kim, Dae Young Yoo, Dae Won Kim, Jung Hoon Choi, and et al. 2020. "Cuprizone Affects Hypothermia-Induced Neuroprotection and Enhanced Neuroblast Differentiation in the Gerbil Hippocampus after Ischemia" Cells 9, no. 6: 1438. https://doi.org/10.3390/cells9061438